Sun Hongliang, Lu Fenghua, Liu Xiaohua, Tian Mingming, Ruan Ziyun, Zhu Peng, Ruan Qiuyan, Jiang Jianrong, Shi Deshun
Guangxi High Education Key Laboratory for Animal Reproduction and Biotechnology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China.
Guangxi High Education Key Laboratory for Animal Reproduction and Biotechnology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China.
Theriogenology. 2015 Apr 15;83(7):1219-25. doi: 10.1016/j.theriogenology.2015.01.003. Epub 2015 Jan 8.
The present study was undertaken to investigate the effect of scriptaid treatment on histone H3 on lysine 18 (H3K18) acetylation and relative expression levels of genes related to histone acetylation (HAT1, CBP, and p300) in buffalo oocytes during IVM. Meanwhile, the embryonic developmental ability of buffalo oocytes after SCNT was also examined. The H3K18 acetylation in oocytes increased from the germinal vesicle (GV) stage to the GV breakdown (GVBD) stage and arrived at a high acetylation level at the GVBD stage. Then, the H3K18 deacetylated completely at the metaphase I (MI) and acetylated again at the MII stage. However, addition of 500-nM scriptaid to the maturation medium resulted in a significant increase in the H3K18 acetylation at the MI stage. The expression profiles of genes related to histone acetylation (HAT1, CBP, and p300) in the meiosis stages of oocytes matured in the medium supplemented with 500-nM scriptaid were significantly higher than those of the oocytes matured in the medium without scriptaid (P < 0.05) with the exception of p300 at the GVBD stage. More SCNT embryos reconstructed with oocytes matured in the medium supplemented with 500-nM scriptaid developed to blastocysts (23.1%) in comparison with oocytes matured in the medium without scriptaid (13.8%, P < 0.05). These results indicate that scriptaid can increase the histone acetylation of buffalo oocytes during meiotic maturation and improve their ability to support the development of SCNT embryos.
本研究旨在探讨曲古抑菌素A处理对水牛卵母细胞体外成熟（IVM）过程中赖氨酸18位点组蛋白H3（H3K18）乙酰化及组蛋白乙酰化相关基因（HAT1、CBP和p300）相对表达水平的影响。同时，还检测了经体细胞核移植（SCNT）后水牛卵母细胞的胚胎发育能力。卵母细胞中的H3K18乙酰化从生发泡（GV）期到生发泡破裂（GVBD）期增加，并在GVBD期达到高乙酰化水平。然后，H3K18在中期I（MI）完全去乙酰化，并在MII期再次乙酰化。然而，在成熟培养基中添加500 nM曲古抑菌素A导致MI期H3K18乙酰化显著增加。在添加500 nM曲古抑菌素A的培养基中成熟的卵母细胞减数分裂阶段，组蛋白乙酰化相关基因（HAT1、CBP和p300）的表达谱显著高于未添加曲古抑菌素A的培养基中成熟的卵母细胞（P < 0.05），GVBD期的p300除外。与在未添加曲古抑菌素A的培养基中成熟的卵母细胞相比，更多用添加500 nM曲古抑菌素A的培养基中成熟的卵母细胞构建的SCNT胚胎发育到囊胚期（23.1%）（13.8%，P < 0.05）。这些结果表明，曲古抑菌素A可以增加水牛卵母细胞减数分裂成熟过程中的组蛋白乙酰化，并提高其支持SCNT胚胎发育的能力。
