Mahmoodi Mohammad M, Rashidian Mohammad, Zhang Yi, Distefano Mark D
Department of Chemistry, University of Minnesota, Minneapolis, Minnesota.
Curr Protoc Protein Sci. 2015 Feb 2;79:15.4.1-15.4.28. doi: 10.1002/0471140864.ps1504s79.
Meta- and para-phenylenediamines have recently been shown to catalyze oxime and hydrazone ligation reactions at rates much faster than aniline, a commonly used catalyst. Here, we demonstrate how these new catalysts can be used in a generally applicable procedure for fluorescent labeling, PEGylation, immobilization, and release of aldehyde- and ketone- functionalized proteins. The chemical orthogonality of phenylenediamine-catalyzed oxime ligation versus copper-catalyzed click reaction has also been harnessed for simultaneous dual labeling of bifunctional proteins containing both aldehyde and alkyne groups in high yield.
间苯二胺和对苯二胺最近被证明能够催化肟和腙连接反应，其速率比常用催化剂苯胺快得多。在此，我们展示了如何将这些新型催化剂用于醛基和酮基功能化蛋白质的荧光标记、聚乙二醇化、固定化及释放的通用方法中。苯二胺催化的肟连接反应与铜催化的点击反应的化学正交性也已被用于高产率地同时对含有醛基和炔基的双功能蛋白质进行双重标记。
