Nasu Michiyo, Takayama Shinichiro, Umezawa Akihiro
Department of Reproductive Biology, National Research Institute for Child Health and Development, Tokyo, Japan.
J Cell Physiol. 2015 Jun;230(6):1376-88. doi: 10.1002/jcp.24882.
The aim of this study is to establish a recapitulation system of human endochondral ossification as a paradigm of developmental engineering. Chondrocytes were isolated from the epiphyseal cartilage of the supernumerary digits of infants with polydactyly. In vivo studies showed that implanted chondrocytes exhibited cartilaginous regeneration over a short period of time and subsequent endochondral ossification with a marrow cavity. Tracing studies revealed that cells of donor origin at the periphery of the cartilage migrated into the center of the cartilage and transformed into osteoblasts, adipocytes, and endothelial cells. Bone marrow was formed through anastomosis with the recipient endothelial system at 13 weeks, and from the migration of recipient hematopoietic cells at 50 weeks. This study provides a human endochondral ossification model system with transdifferentiation of the donor cells at the periphery of the cartilage. J. Cell. Physiol. 230: 1376-1388, 2015. © 2015 Wiley Periodicals, Inc., A Wiley Company.
本研究的目的是建立一个人类软骨内成骨的重现系统，作为发育工程的范例。从多指婴儿多余手指的骨骺软骨中分离软骨细胞。体内研究表明，植入的软骨细胞在短时间内表现出软骨再生，随后进行软骨内成骨并形成骨髓腔。追踪研究显示，软骨周边供体来源的细胞迁移到软骨中心并转化为成骨细胞、脂肪细胞和内皮细胞。骨髓在13周时通过与受体内皮系统吻合形成，在50周时通过受体造血细胞迁移形成。本研究提供了一个人类软骨内成骨模型系统，其中软骨周边的供体细胞发生了转分化。《细胞生理学杂志》230: 1376 - 1388, 2015。© 2015威利期刊公司，威利公司旗下。
