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使用标准清洁方法从医院常见表面、玩具和书籍上去除花生过敏原Ara h 1。

Removal of peanut allergen Ara h 1 from common hospital surfaces, toys and books using standard cleaning methods.

作者信息

Watson Wade Ta, Woodrow AnnMarie, Stadnyk Andrew W

机构信息

Department of Pediatrics, Dalhousie University, Halifax, NS Canada ; IWK Health Centre, 5850/5980 University Avenue, Halifax, NS Canada B3K 6R8.

IWK Health Centre, 5850/5980 University Avenue, Halifax, NS Canada B3K 6R8.

出版信息

Allergy Asthma Clin Immunol. 2015 Jan 23;11(1):4. doi: 10.1186/s13223-015-0069-x. eCollection 2015.

Abstract

BACKGROUND

In children, a diagnosis of peanut allergy causes concern about accidental exposure because even small amounts of peanut protein could trigger an allergic reaction. Contamination of toys, books or other items by peanut butter in areas where individuals have eaten may occur in hospital waiting rooms and cafeterias. It is not known if hospital cleaning wipes are effective in removing peanut allergen.

OBJECTIVES

The purpose of this study was to determine whether cleaning peanut contaminated items with common household and hospital cleaning wipes would remove peanut allergen.

METHODS

5 mL of peanut butter was evenly smeared on a 12 inch by 12 inch (30.5 by 30.5 cm) square on a nonporous (laminated plastic) table surface, a plastic doll, and a textured plastic ball, and 2.5 mL was applied to smooth and textured book covers. Samples for measurement of Ara h 1 were collected prior to the application of the peanut butter (baseline), and after cleaning with a common household wipe and two commercial hospital wipes. A monoclonal-based ELISA for arachis hypogaea allergen 1 (Ara h 1), range of detection 1.95-2000 ng/mL, was used to assess peanut allergen on each item. The samples were diluted 1:50 for testing.

RESULTS

At baseline, there was no detectable Ara h 1 allergen on any item at baseline. Detectable Ara h 1 was detected on all products after applying peanut butter (range 1.2-19.0 micrograms/mL). After cleaning with any product, no Ara h 1 was detected on any item.

CONCLUSIONS

Table surfaces, book covers and plastic toys can be cleaned to remove peanut allergen Ara h 1 using common household and hospital cleaning wipes. Regular cleaning of these products or cleaning prior to their use should be promoted to reduce the risk of accidental peanut exposure, especially in areas where they have been used by many children.

摘要

背景

在儿童中,花生过敏的诊断引发了对意外接触的担忧,因为即使少量的花生蛋白也可能引发过敏反应。在医院候诊室和自助餐厅等人们进食的区域,玩具、书籍或其他物品可能会被花生酱污染。目前尚不清楚医院清洁湿巾是否能有效去除花生过敏原。

目的

本研究的目的是确定使用普通家用和医院清洁湿巾清洁被花生污染的物品是否能去除花生过敏原。

方法

将5毫升花生酱均匀涂抹在无孔(层压塑料)桌面、塑料玩偶和有纹理的塑料球的12英寸×12英寸(30.5×30.5厘米)正方形区域上,并将2.5毫升花生酱涂抹在光滑和有纹理的书皮上。在涂抹花生酱之前(基线)以及用普通家用湿巾和两种商用医院湿巾清洁后,收集用于测量Ara h 1的样本。使用基于单克隆抗体的花生过敏原1(Ara h 1)酶联免疫吸附测定法(检测范围为1.95 - 2000纳克/毫升)评估每个物品上的花生过敏原。样本稀释1:50进行检测。

结果

在基线时,任何物品上均未检测到Ara h 1过敏原。涂抹花生酱后,所有产品上均检测到可检测到的Ara h 1(范围为1.2 - 19.0微克/毫升)。用任何产品清洁后,任何物品上均未检测到Ara h 1。

结论

使用普通家用和医院清洁湿巾可以清洁桌面、书皮和塑料玩具,以去除花生过敏原Ara h 1。应提倡定期清洁这些产品或在使用前进行清洁,以降低意外接触花生的风险,尤其是在许多儿童使用过的区域。

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