Jafari Mahvash, Shirbazou Shanaz, Norozi Majid
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Parasitology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2014 Mar;9(1):60-9.
The aim of this study was to investigate the effects of Leishmania major infection on the induction of oxidative stress in skin and lung of female mice.
BALB/c mice were randomly divided into the control and experimental groups. The experimental groups were subcutaneously infected with inoculums promastigotes of L. major. The animals were sacrificed at 20, 40, 60, 90 and 120 days post-infection, and tissues were isolated and analyzed.
Superoxide dismutase activity, percent of DNA fragmentation and superoxide anion production levels were increased in skin and lung of infected mice. Lung catalase activity and skin malondialdehyde level were also increased. The decreased glutathione level was observed in both tissues. The highest alteration in these parameters in both tissues was observed at 90 days post-infection.
L. major infection induces the production of free radicals and oxidative stress in a time-dependent manner in mice skin and lung by depletion of glutathione and increasing lipid peroxidation. The elevated DNA fragmentation may be related with increased oxidative stress. The skin is more sensitive to the effects of L. major infection on oxidative stress induction compared to the lung.
本研究旨在探讨硕大利什曼原虫感染对雌性小鼠皮肤和肺部氧化应激诱导的影响。
将BALB/c小鼠随机分为对照组和实验组。实验组皮下接种硕大利什曼原虫前鞭毛体接种物。在感染后20、40、60、90和120天处死动物，分离并分析组织。
感染小鼠的皮肤和肺部超氧化物歧化酶活性、DNA片段化百分比和超氧阴离子产生水平均升高。肺部过氧化氢酶活性和皮肤丙二醛水平也升高。在两个组织中均观察到谷胱甘肽水平降低。在感染后90天观察到两个组织中这些参数的最高变化。
硕大利什曼原虫感染通过消耗谷胱甘肽和增加脂质过氧化作用，以时间依赖性方式在小鼠皮肤和肺部诱导自由基产生和氧化应激。DNA片段化增加可能与氧化应激增加有关。与肺部相比皮肤对硕大利什曼原虫感染诱导氧化应激的影响更敏感。
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