Liao Che-Yang, Smet Wouter, Brunoud Geraldine, Yoshida Saiko, Vernoux Teva, Weijers Dolf
Laboratory of Biochemistry, Wageningen University, Wageningen, the Netherlands.
Laboratoire de Reproduction et Développement des Plantes, CNRS, Institut National de la Recherche Agronomique, Ecole Normale Superieure Lyon, Lyon, France.
Nat Methods. 2015 Mar;12(3):207-10, 2 p following 210. doi: 10.1038/nmeth.3279. Epub 2015 Feb 2.
The visualization of hormonal signaling input and output is key to understanding how multicellular development is regulated. The plant signaling molecule auxin triggers many growth and developmental responses, but current tools lack the sensitivity or precision to visualize these. We developed a set of fluorescent reporters that allow sensitive and semiquantitative readout of auxin responses at cellular resolution in Arabidopsis thaliana. These generic tools are suitable for any transformable plant species.
激素信号输入和输出的可视化是理解多细胞发育如何调控的关键。植物信号分子生长素引发许多生长和发育反应,但目前的工具缺乏可视化这些反应的灵敏度或精度。我们开发了一套荧光报告基因,可在细胞分辨率下对拟南芥中的生长素反应进行灵敏且半定量的读出。这些通用工具适用于任何可转化的植物物种。