一种肌肉乙酰胆碱受体在人小脑髓母细胞瘤细胞系TE671中表达。
A muscle acetylcholine receptor is expressed in the human cerebellar medulloblastoma cell line TE671.
作者信息
Luther M A, Schoepfer R, Whiting P, Casey B, Blatt Y, Montal M S, Montal M, Linstrom J
机构信息
Salk Institute for Biological Studies, San Diego, California 92138.
出版信息
J Neurosci. 1989 Mar;9(3):1082-96. doi: 10.1523/JNEUROSCI.09-03-01082.1989.
The human neuromedulloblastoma cell line TE671 is shown by single-channel recordings to express nicotinic acetylcholine receptors (AChRs) that are blocked by alpha-bungarotoxin (alpha Bgt). These AChRs do not react with antisera to the alpha Bgt-binding protein of brain or with monoclonal antibodies (mAbs) to brain nicotinic AChRs that do not bind alpha Bgt. TE671 AChRs do react with autoantibodies to muscle AChRs from myasthenia gravis patients and with mAbs to muscle AChRs, including mAbs specific for extrajunctional AChRs. AChRs. AChRs purified from TE671 cells are composed of 4 kinds of subunits corresponding to those of muscle AChR. Sequences of cDNAs for the ACh-binding alpha subunit and the delta subunit of this AChR further identify it as muscle AChR. Expression of TE671 AChR can be up-regulated by nicotine and dexamethasone, and down-regulated by forskolin.
单通道记录显示，人神经母细胞瘤细胞系TE671表达可被α-银环蛇毒素（αBgt）阻断的烟碱型乙酰胆碱受体（AChRs）。这些AChRs不与针对脑αBgt结合蛋白的抗血清反应，也不与针对不结合αBgt的脑烟碱型AChRs的单克隆抗体（mAbs）反应。TE671 AChRs确实与重症肌无力患者肌肉AChRs的自身抗体以及肌肉AChRs的mAbs反应，包括对接头外AChRs特异的mAbs。从TE671细胞纯化的AChRs由4种与肌肉AChR相应的亚基组成。该AChR的ACh结合α亚基和δ亚基的cDNA序列进一步将其鉴定为肌肉AChR。TE671 AChR的表达可被尼古丁和地塞米松上调，并被福斯可林下调。
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