淋巴细胞中电压门控钠通道的鉴定与功能特性研究
Identification and functional characterization of voltage-gated sodium channels in lymphocytes.
作者信息
Huang Weifeng, Lu Chunjing, Wu Yong, Ouyang Shou, Chen Yuanzhong
机构信息
Fujian Institute of Hematology, Fujian Medical University Union Hospital, Fuzhou 350004, PR China.
Department of Blood Transfusion, Maternal and Child Health Hospital of Xiamen, Xiamen 361003, PR China.
出版信息
Biochem Biophys Res Commun. 2015 Mar 6;458(2):294-9. doi: 10.1016/j.bbrc.2015.01.103. Epub 2015 Jan 31.
A variety of ion channels has been discovered in lymphocytes. RT-PCR and real-time PCR analysis revealed that ALL (acute lymphocytic leukemia) cell lines and human peripheral blood mononuclear cells mainly expressed TTX (tetrodotoxin)-sensitive voltage-gated sodium channels (VGSCs). Expression of VGSC protein was confirmed by western blots and Immunofluorescence. Whole-cell patch-clamp recordings showed that a sub-population (20%) of MOLT-4 cells expressed functional VGSCs, which were TTX-sensitive. Importantly, 2 μM TTX decreased the invasion of Jurkat and MOLT-4 cells ∼90%. These results indicate that the activity of VGSCs could represent a novel mechanism potentiating the invasive capacity of these cells.
在淋巴细胞中发现了多种离子通道。逆转录聚合酶链反应（RT-PCR）和实时定量聚合酶链反应（real-time PCR）分析显示，急性淋巴细胞白血病（ALL）细胞系和人外周血单个核细胞主要表达对河豚毒素（TTX）敏感的电压门控钠通道（VGSCs）。通过蛋白质免疫印迹法（western blots）和免疫荧光法证实了VGSC蛋白的表达。全细胞膜片钳记录显示，MOLT-4细胞亚群（20%）表达功能性VGSCs，且对TTX敏感。重要的是，2 μM TTX使Jurkat细胞和MOLT-4细胞的侵袭能力降低了约90%。这些结果表明，VGSCs的活性可能代表了增强这些细胞侵袭能力的一种新机制。
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