通过聚合酶链反应在来自美国健康献血者的血浆以及来自喀麦隆多次输血患者的血清中未检测到巨血型马赛样病毒DNA。
Absence of giant blood Marseille-like virus DNA detection by polymerase chain reaction in plasma from healthy US blood donors and serum from multiply transfused patients from Cameroon.
作者信息
Phan Tung Gia, Desnues Christelle, Switzer William M, Djoko Cyrille F, Schneider Bradley S, Deng Xutao, Delwart Eric
机构信息
Blood Systems Research Institute.
Department of Laboratory Medicine, University of California at San Francisco, San Francisco, California.
出版信息
Transfusion. 2015 Jun;55(6):1256-62. doi: 10.1111/trf.12997. Epub 2015 Feb 2.
BACKGROUND
A new Marseilleviridae virus family member, giant blood Marseille-like (GBM) virus, was recently reported in persons from France in the serum of an infant with adenitis, in the blood of 4% of healthy blood donors, and in 9% of multiply transfused thalassemia patients. These results suggested the presence of a nucleocytoplasmic large DNA virus potentially transmissible by blood product transfusion.
STUDY DESIGN AND METHODS
To investigate this possibility we tested the plasma from 113 US blood donors and 74 multiply transfused Cameroon patients for GBM viral DNA using highly sensitive polymerase chain reaction (PCR) assays.
RESULTS
GBM DNA was not detected by nested PCR in any of these 187 human specimens.
CONCLUSIONS
Further testing is required to confirm the occurrence of human GBM virus infections.
背景
最近在法国人的血清中发现了马赛病毒科的一个新成员，即巨血马赛样（GBM）病毒，该病毒存在于一名患腺炎婴儿的血清中、4%的健康献血者血液中以及9%多次输血的地中海贫血患者血液中。这些结果表明存在一种可能通过输血传播的核质大DNA病毒。
研究设计与方法
为了研究这种可能性，我们使用高灵敏度聚合酶链反应（PCR）检测法，对113名美国献血者的血浆和74名喀麦隆多次输血患者的血浆进行了GBM病毒DNA检测。
结果
在这187份人类样本中，巢式PCR均未检测到GBM DNA。
结论
需要进一步检测以确认人类GBM病毒感染的发生情况。
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