Fan Yong, Zhao Hong-Cui, Liu Jianqiao, Tan Tao, Ding Ting, Li Rong, Zhao Yue, Yan Jie, Sun Xiaofang, Yu Yang, Qiao Jie
Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
1] Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China [2] Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China [3] Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
Sci Rep. 2015 Feb 3;5:8192. doi: 10.1038/srep08192.
Fertilisation is the first step in embryonic development, and dynamic changes of key genes may potentially improve assisted reproduction techniques efficiency during this process. Here, we analysed genes that were differentially expressed between oocytes and zygotes and focused on cytokinesis-related genes. Plk1 and Dctn3 were identified as showing dramatic changes in expression during fertilisation and were suggested to play a key role in inducing aneuploidy in zygotes and 8-cell embryos. Moreover, we found that maternal Plk1 and Dctn3 were expressed at lower levels in in vitro matured oocytes, which may have contributed to the high ratio of resulting embryos with abnormal Plk1 and Dctn3 expression levels, thereby reducing the developmental competence of the resulting embryos. Furthermore, the overexpression of Dctn3 can silence Plk1 expression, which suggests a potential regulation mechanism. In conclusion, our present study showed that aberrant expression of Plk1 and Dctn3 increases embryo aneuploidy and developmental failure, particularly in in vitro matured oocytes. Our results facilitate a better understanding of the effects of oocyte maternal gene expression on embryonic development and can be used to improve the outcome of assisted reproduction techniques.
受精是胚胎发育的第一步，在此过程中关键基因的动态变化可能会提高辅助生殖技术的效率。在这里，我们分析了卵母细胞和受精卵之间差异表达的基因，并重点关注了与胞质分裂相关的基因。Plk1和Dctn3被确定为在受精过程中表达有显著变化，并被认为在受精卵和8细胞胚胎中诱导非整倍体方面起关键作用。此外，我们发现母体Plk1和Dctn3在体外成熟的卵母细胞中表达水平较低，这可能导致了所得胚胎中Plk1和Dctn3表达水平异常的比例较高，从而降低了所得胚胎的发育能力。此外，Dctn3的过表达可以使Plk1表达沉默，这表明存在一种潜在的调控机制。总之，我们目前的研究表明，Plk1和Dctn3的异常表达会增加胚胎非整倍体和发育失败的发生率，尤其是在体外成熟的卵母细胞中。我们的结果有助于更好地理解卵母细胞母体基因表达对胚胎发育的影响，并可用于改善辅助生殖技术的结果。
