Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
BMB Rep. 2010 Dec;43(12):818-23. doi: 10.5483/BMBRep.2010.43.12.818.
Plk 1 is overexpressed in many human malignancies including laryngeal carcinoma. However, its therapeutic potential has been never examined in laryngeal carcinoma. In the present study, a simple cellular morphology-based strategy was firstly proposed for rapidly screening the effective siRNAs against Plk1. Furthermore, we investigated the effects of Plk1 depletion via a novel identified effective siRNA against Plk1, Plk1 siRNA-607, on human laryngeal carcinoma Hep-2 cells. The results indicated that Plk1 siRNA-607 transfection resulted in a significant inhibition in Plk1 expression in cells, and subsequently caused a dramatic mitotic cell cycle arrest followed by massive apoptotic cell death, and eventually resulted in a significant decrease in growth and viability of the laryngeal carcinoma cells. Taken together, our present study not only suggests a simple strategy for rapidly screening effective siRNAs against Plk1 but also implicates that Plk1 may serve as a potential therapeutic target in human laryngeal carcinoma.
Plk1 在许多人类恶性肿瘤中过表达，包括喉癌。然而，其在喉癌中的治疗潜力从未被研究过。在本研究中，我们首次提出了一种基于简单细胞形态学的策略，用于快速筛选针对 Plk1 的有效 siRNA。此外，我们通过一种新鉴定的针对 Plk1 的有效 siRNA（Plk1 siRNA-607）来研究 Plk1 耗竭对人喉癌细胞 Hep-2 的影响。结果表明，Plk1 siRNA-607 转染导致细胞中 Plk1 表达显著抑制，随后导致有丝分裂细胞周期明显停滞，随后大量发生细胞凋亡，最终导致喉癌细胞的生长和活力显著下降。综上所述，本研究不仅提出了一种快速筛选针对 Plk1 的有效 siRNA 的简单策略，还表明 Plk1 可能成为人类喉癌的一个潜在治疗靶点。
