Jeftinija S
Department of Veterinary Anatomy, Iowa State University, Ames 50011.
Neurosci Lett. 1989 Jan 16;96(2):191-6. doi: 10.1016/0304-3940(89)90056-6.
The role of subtypes of excitatory amino acid receptor in synaptic transmission in the spinal dorsal horn has been studied in an in vitro slice preparation with well-preserved afferent inputs to the dorsal horn. Intracellular recordings were made from 30 dorsal horn neurons in laminae I-III of 18-28 day old rats. Superfusion of the slice with a Mg2+ zero solution resulted in an increase in the amplitude and duration of dorsal root-evoked excitatory postsynaptic potentials (EPSP) recorded intracellularly from dorsal horn neurons. Bath application of D-2-amino-5-phosphonovalerate (10(-5) M to 2.5 x 10(-5) M) or DL-2-amino-5-phosphonovalerate (10(-4) M to 2.5 x 10(-4) M) rapidly and reversibly abolished the later component of the EPSP evoked by activation of either group of primary afferents and selectively antagonized the N-methyl-D-aspartate-induced depolarization.
在一种保留了完好的背角传入输入的体外脑片标本中，研究了兴奋性氨基酸受体亚型在脊髓背角突触传递中的作用。对18 - 28日龄大鼠I - III层的30个背角神经元进行了细胞内记录。用Mg2+零溶液灌流脑片，导致从背角神经元细胞内记录到的背根诱发兴奋性突触后电位（EPSP）的幅度和时程增加。浴槽中加入D - 2 - 氨基 - 5 - 磷酸戊酸（10^(-5) M至2.5×10^(-5) M）或DL - 2 - 氨基 - 5 - 磷酸戊酸（10^(-4) M至2.5×10^(-4) M）可迅速且可逆地消除由任一组初级传入纤维激活所诱发的EPSP的后期成分，并选择性地拮抗N - 甲基 - D - 天冬氨酸诱导的去极化。
