Lee Sanghee, Yang Guang, Bushman Wade
Department of Urology, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin, United States of America; Cellular and Molecular Biology Program, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin, United States of America.
Department of Urology, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin, United States of America.
PLoS One. 2015 Feb 3;10(2):e0116827. doi: 10.1371/journal.pone.0116827. eCollection 2015.
Lower urinary tract symptoms (LUTS) including urinary frequency and nocturia are common in aging men. Recent studies have revealed a strong association of prostatic inflammation with LUTS. We developed an animal model of bacterial induced, isolated prostatic inflammation and examined the effect of prostatic inflammation on voiding behavior in adult C57BL/6J mice. Prostatic inflammation was induced by transurethral inoculation of uropathogenic E. coli-1677. Bacterial cystitis was prevented by continuous administration of nitrofurantoin. Hematoxylin and eosin (H&E) staining and bacterial culture were preformed to validate our animal model. Voiding behavior was examined by metabolic cage testing on post-instillation day 1 (PID 1), PID 4, PID 7 and PID 14 and both voiding frequency and volume per void were determined. Mice with prostatic inflammation showed significantly increased voiding frequency at PID 1, 7 and 14, and decreased volume per void at all time points, as compared to mice instilled with saline and receiving nitrofurantoin (NTF). Linked analysis of voiding frequency and voided volumes revealed an overwhelming preponderance of high frequency, low volume voiding in mice with prostatic inflammation. These observations suggest that prostatic inflammation may be causal for symptoms of urinary frequency and nocturia.
包括尿频和夜尿症在内的下尿路症状(LUTS)在老年男性中很常见。最近的研究表明前列腺炎症与LUTS之间存在密切关联。我们建立了一种细菌诱导的孤立性前列腺炎症动物模型,并研究了前列腺炎症对成年C57BL/6J小鼠排尿行为的影响。通过经尿道接种尿路致病性大肠杆菌-1677诱导前列腺炎症。通过持续给予呋喃妥因预防细菌性膀胱炎。进行苏木精和伊红(H&E)染色及细菌培养以验证我们的动物模型。在接种后第1天(PID 1)、PID 4、PID 7和PID 14通过代谢笼试验检查排尿行为,并确定排尿频率和每次排尿量。与注入生理盐水并接受呋喃妥因(NTF)的小鼠相比,患有前列腺炎症的小鼠在PID 1、7和14时排尿频率显著增加,且在所有时间点每次排尿量均减少。对排尿频率和排尿量的关联分析显示,患有前列腺炎症的小鼠中高频、低量排尿占压倒性优势。这些观察结果表明前列腺炎症可能是尿频和夜尿症症状的病因。