Department of Urology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53792, USA.
Prostate. 2012 Feb;72(3):307-17. doi: 10.1002/pros.21433. Epub 2011 Jun 16.
Prostatic inflammation is gaining increasing attention as a potential etiologic factor in prostate cancer, benign prostatic hyperplasia, lower urinary tract symptoms, and CPPS. This study was performed to address the need for a well characterized model of acute prostatic inflammation that may be used to study the effect of acute inflammation on epithelial and stromal cell proliferation, voiding behavior, and neurovascular physiology.
Uropathogenic E. coli 1677 was instilled transurethrally into adult C57BL/6J male mice. Prostates were analyzed at 1, 2, 3, 5, 7, or 14 days post-instillation and compared to saline-instilled and naïve controls. Time course and severity of inflammation were characterized by the quantity and type of inflammatory infiltrate present, hemorrhage, proliferation, and reactive hyperplasia. RT-PCR was performed to characterize inflammatory mediators including IL-1α, IL-1β, IL-1RA, IL-18, IL-6, IL-10, IL-8, TNFα, and COX-2.
Inflammation was evident in all lobes of the prostate with the DLP most severely affected. Infection consistently led to a significant increase in neutrophils and macrophages in the early stages of prostate infection, followed by lymphocytic inflammation at the later time points. Inflammation was accompanied by induction of several inflammatory genes, including IL-1 family members, IL-6, and COX-2, and induced a significant increase in epithelial proliferation and reactive hyperplasia in all three prostate lobes.
Transurethral inoculation of uropathogenic E. coli 1677 reliably infects the mouse prostate, produces a significant inflammatory response, and induces quantifiable epithelial proliferation and reactive hyperplasia.
前列腺炎症作为前列腺癌、良性前列腺增生、下尿路症状和 CPPS 的潜在病因正受到越来越多的关注。本研究旨在建立一种特征明确的急性前列腺炎症模型，用于研究急性炎症对上皮和基质细胞增殖、排尿行为和神经血管生理学的影响。
通过经尿道将尿路致病性大肠杆菌 1677 注入成年 C57BL/6J 雄性小鼠体内。在注入后 1、2、3、5、7 或 14 天分析前列腺，并与生理盐水注入和未处理的对照组进行比较。通过存在的炎症浸润物的数量和类型、出血、增殖和反应性增生来描述炎症的时程和严重程度。通过 RT-PCR 来描述炎症介质，包括 IL-1α、IL-1β、IL-1RA、IL-18、IL-6、IL-10、IL-8、TNFα 和 COX-2。
在前列腺的所有叶中都可见炎症，其中 DLP 受影响最严重。感染始终导致前列腺感染早期阶段中性粒细胞和巨噬细胞显著增加，随后在后期阶段出现淋巴细胞炎症。炎症伴随着几个炎症基因的诱导，包括 IL-1 家族成员、IL-6 和 COX-2，并导致所有三个前列腺叶的上皮增殖和反应性增生显著增加。
经尿道接种尿路致病性大肠杆菌 1677 可可靠地感染小鼠前列腺，引起明显的炎症反应，并诱导可量化的上皮增殖和反应性增生。
