Papiez Monika A, Krzyściak Wirginia
Department of Cytobiology, Jagiellonian University Medical College, Krakow, Poland.
Folia Med Cracov. 2014;54(2):71-9.
The effect of curcumin, a promising anticancer agent, on the action of certain cytostatic drugs, including etoposide, is not well understood. This paper examines the effect of curcumin on etoposide action in leukemic and normal bone marrow cells in vivo conditions. The experimental model used was Brown Norway rats with a transplantable acute promyelocytic leukemia. Leukemia was induced by intravenous injection of BNML (Brown Norway Myeloid Leukemia) cells. Curcumin was administered by oral gavage (200 mg/kg) for 23 consecutive days and etoposide was used intraperitoneally (50 mg/kg) for the last three days of the experiment. Control leukemic and healthy rats received the solvent for the tested compounds only. Curcumin significantly reduced the number of leukemic promyelocytes in the bone marrow of BNML rats in comparison to the leukemic control. Treatment with curcumin plus etoposide led to a decrease in the number of promyelocytes to the normal values occurring in healthy individuals. In contrast, the percentage of the normal precursors of granulocytes (p <0.001) and erythrocytes (p <0.001) increased significantly in comparison to the group treated with only etoposide. The results of the study indicate that curcumin may protect healthy myeloid cells against the cytotoxic effect of etoposide and potentiate the antileukemic action of this anticancer drug.
姜黄素是一种很有前景的抗癌药物，其对包括依托泊苷在内的某些细胞毒性药物作用的影响尚未完全明确。本文研究了姜黄素在体内条件下对白血病细胞和正常骨髓细胞中依托泊苷作用的影响。所采用的实验模型是患有可移植性急性早幼粒细胞白血病的棕色挪威大鼠。通过静脉注射BNML（棕色挪威髓性白血病）细胞诱导白血病。连续23天经口灌胃给予姜黄素（200mg/kg），并在实验的最后三天腹腔注射依托泊苷（50mg/kg）。对照白血病大鼠和健康大鼠仅接受受试化合物的溶剂。与白血病对照组相比，姜黄素显著减少了BNML大鼠骨髓中白血病早幼粒细胞的数量。姜黄素加依托泊苷治疗使早幼粒细胞数量降至健康个体中的正常水平。相比之下，与仅接受依托泊苷治疗的组相比，粒细胞（p<0.001）和红细胞（p<0.001）的正常前体细胞百分比显著增加。研究结果表明，姜黄素可能保护健康的髓细胞免受依托泊苷的细胞毒性作用，并增强这种抗癌药物的抗白血病作用。
