Department of Cytobiology, Pharmaceutical Faculty, Jagiellonian University Medical College, Medyczna 9 Str., 30-688 Kraków, Poland.
Anticancer Res. 2012 Jul;32(7):2905-13.
(-)-Epicatechin (EC) is a naturally occurring compound which induces oxidative DNA damage in human acute myeloid leukaemia (AML) cells.
The aim of the study was to examine the influence of EC on the antileukaemic effect of etoposide in rats with AML.
Brown Norway rats with AML were treated with EC for 23 days and etoposide was administered for the last three days of the experiment. Bone marrow and splenic cell apoptosis was investigated by flow cytometry using annexin V-allophycocyanin staining. The oxidative status was investigated in homogenates of the liver.
EC was found to increase the in vivo apoptotic effect of etoposide resulting in the decrease of the percentage of leukaemia cells in EC-treated rats in comparison to those treated with etoposide only. Investigation of malondialdehyde and ferric ion-reducing ability of plasma levels indicated that EC increases the oxidative stress induced by etoposide in leukaemic rats.
EC can enhance the antileukaemia properties of etoposide in vivo through augmentation of oxidative stress.
(-)-表儿茶素（EC）是一种天然存在的化合物，可诱导人急性髓系白血病（AML）细胞发生氧化 DNA 损伤。
本研究旨在探讨 EC 对 AML 大鼠依托泊苷抗白血病作用的影响。
采用 AML 棕色挪威大鼠，用 EC 处理 23 天，并在实验的最后三天给予依托泊苷。采用 Annexin V-藻红蛋白染色，通过流式细胞术检测骨髓和脾细胞凋亡。在肝匀浆中检测氧化状态。
发现 EC 可增强依托泊苷的体内促凋亡作用，与仅用依托泊苷治疗的大鼠相比，EC 治疗的大鼠白血病细胞比例下降。对丙二醛和血浆铁还原能力的检测表明，EC 增加了依托泊苷诱导的白血病大鼠的氧化应激。
EC 可通过增强氧化应激增强依托泊苷在体内的抗白血病作用。
