Saito Hikaru, Oikawa Takaya, Hamamoto Shin, Ishimaru Yasuhiro, Kanamori-Sato Miyu, Sasaki-Sekimoto Yuko, Utsumi Tomoya, Chen Jing, Kanno Yuri, Masuda Shinji, Kamiya Yuji, Seo Mitsunori, Uozumi Nobuyuki, Ueda Minoru, Ohta Hiroyuki
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B65 Nagatsuta-cho Midori-ku, Yokohama 226-8501, Japan.
Graduate School of Science, Tohoku University, 6-3, Aramaki-Aza-Aoba, Aoba-ku, Sendai 980-0845, Japan.
Nat Commun. 2015 Feb 4;6:6095. doi: 10.1038/ncomms7095.
Plant hormones are transported across cell membranes during various physiological events. Recent identification of abscisic acid and strigolactone transporters suggests that transport of various plant hormones across membranes does not occur by simple diffusion but requires transporter proteins that are strictly regulated during development. Here, we report that a major glucosinolate transporter, GTR1/NPF2.10, is multifunctional and may be involved in hormone transport in Arabidopsis thaliana. When heterologously expressed in oocytes, GTR1 transports jasmonoyl-isoleucine and gibberellin in addition to glucosinolates. gtr1 mutants are severely impaired in filament elongation and anther dehiscence resulting in reduced fertility, but these phenotypes can be rescued by gibberellin treatment. These results suggest that GTR1 may be a multifunctional transporter for the structurally distinct compounds glucosinolates, jasmonoyl-isoleucine and gibberellin, and may positively regulate stamen development by mediating gibberellin supply.
在各种生理过程中，植物激素会跨细胞膜运输。最近对脱落酸和独脚金内酯转运蛋白的鉴定表明，各种植物激素跨膜运输并非通过简单扩散进行，而是需要在发育过程中受到严格调控的转运蛋白。在此，我们报告一种主要的硫代葡萄糖苷转运蛋白GTR1/NPF2.10具有多种功能，可能参与拟南芥中的激素运输。当在卵母细胞中异源表达时，GTR1除了转运硫代葡萄糖苷外，还转运茉莉酰异亮氨酸和赤霉素。gtr1突变体在花丝伸长和花药开裂方面严重受损，导致育性降低，但这些表型可通过赤霉素处理得到挽救。这些结果表明，GTR1可能是一种针对结构不同的化合物硫代葡萄糖苷、茉莉酰异亮氨酸和赤霉素的多功能转运蛋白，并且可能通过介导赤霉素供应来正向调节雄蕊发育。
