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下一代测序技术获得了帝王神仙鱼(Pygoplites diacanthus,鲈形目:棘蝶鱼科)的完整线粒体基因组。

Next generation sequencing yields the complete mitochondrial genome of the Regal angelfish, Pygoplites diacanthus (Perciformes: Pomacanthidae).

作者信息

Shen Kang-Ning, Chang Chih-Wei, Chen Ching-Hung, Hsiao Chung-Der

机构信息

a Center of Excellence for the Oceans, National Taiwan Ocean University , Keelung , Taiwan.

b National Museum of Marine Biology and Aquarium , Checheng , Pingtung , Taiwan.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4149-4150. doi: 10.3109/19401736.2014.1003886. Epub 2015 Feb 4.

Abstract

In this study, the complete mitogenome sequence of the Regal angelfish, Pygoplites diacanthus (Perciformes: Pomacanthidae) has been sequenced by the next-generation sequencing method. The assembled mitogenome consisting of 16,784 bp includes 13 protein coding genes, 22 transfer RNAs, and two ribosomal RNAs genes. The overall base composition of Regal angelfish is 28.5% for A, 28.9% for C, 16.3% for G, 26.4% for T and show 85% identities to flame angelfish Centropyge loricula. The complete mitogenome of the Regal angelfish provides essential and important DNA molecular data for further phylogeography and evolutionary analysis for marine angelfish phylogeny.

摘要

在本研究中,通过下一代测序方法对女王神仙(Pygoplites diacanthus,鲈形目:棘蝶鱼科)的完整线粒体基因组序列进行了测序。组装后的线粒体基因组由16,784个碱基对组成,包括13个蛋白质编码基因、22个转运RNA和两个核糖体RNA基因。女王神仙的总体碱基组成为:A占28.5%,C占28.9%,G占16.3%,T占26.4%,与火焰神仙(Centropyge loricula)的序列一致性为85%。女王神仙的完整线粒体基因组为海洋神仙鱼系统发育的进一步系统地理学和进化分析提供了重要的DNA分子数据。

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