Shen Kang-Ning, Chang Chih-Wei, Chan Yen-Fan, Lin Zi-Han, Tsai Shiou-Yi, Chen Ching-Hung, Hsiao Chung-Der
a Center of Excellence for the Oceans, National Taiwan Ocean University , Keelung , Taiwan .
b National Museum of Marine Biology and Aquarium , Pingtung , Taiwan .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3672-3. doi: 10.3109/19401736.2015.1079849. Epub 2015 Sep 2.
In this study, we used the next-generation sequencing method to deduce two complete mitogenomes of Woodhead's angelfish (Centropyge woodheadi) and Herald's angelfish (Centropyge heraldi) for the first time. The assembled mitogenome, consisting of 16 863 and 16 836 bp, shows 95.3% identity to each other. Both mitogenome follow a typical vertebrate arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop contains 1158 bp in C. woodheadi and 1129 bp in C. heraldi and locates between tRNA-Pro and tRNA-Phe. The overall GC content is 47.9% for C. woodheadi and 47.5% for C. heraldi. The complete mitogenomes of C. woodheadi and C. heraldi provide an essential and important DNA molecular data for phylogenetic and evolutionary analysis for marine angelfish.
在本研究中，我们首次使用下一代测序方法推导得出伍德黑德神仙鱼（Centropyge woodheadi）和赫勒尔德神仙鱼（Centropyge heraldi）的两个完整线粒体基因组。组装后的线粒体基因组分别由16863 bp和16836 bp组成，彼此间具有95.3%的同源性。两个线粒体基因组均遵循典型的脊椎动物排列方式，包括13个蛋白质编码基因、22个转运RNA、2个核糖体RNA基因以及一个D-loop非编码控制区。伍德黑德神仙鱼的D-loop包含1158 bp，赫勒尔德神仙鱼的D-loop包含1129 bp，且均位于tRNA-Pro和tRNA-Phe之间。伍德黑德神仙鱼的总体GC含量为47.9%，赫勒尔德神仙鱼的总体GC含量为47.5%。伍德黑德神仙鱼和赫勒尔德神仙鱼的完整线粒体基因组为海水神仙鱼的系统发育和进化分析提供了重要的DNA分子数据。
