Honjo Hiroshi, Tsuruno Keigo, Tatsuke Tsuneyuki, Sato Masaki, Hanai Taizo
Laboratory for Bioinformatics, Graduate School of Systems Life Sciences, Kyushu University, 804 Westwing, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Laboratory for Bioinformatics, Graduate School of Systems Life Sciences, Kyushu University, 804 Westwing, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
J Biosci Bioeng. 2015 Aug;120(2):199-204. doi: 10.1016/j.jbiosc.2014.12.023. Epub 2015 Jan 30.
3-Hydroxypropionic acid (3-HP) is an important platform C3 chemical; production of 3-HP in recombinant Escherichia coli by synthetic pathways has been the focus of a lot of research. When glycerol is used as a substrate to produce 3-HP in E. coli, only the ALDH pathway (employing aldehyde dehydrogenase (ALDH) for conversion of 3-hydroxypropionaldehyde (3-HPA) into 3-HP) has been utilized as a synthetic pathway. However, several bacteria (including Klebsiella pneumoniae) are known to have the ability to produce 3-HP by the Pdu pathway (employing the PduP, PduL, and PduW enzymes). Here, we report the production of 3-HP in E. coli by using the Pdu pathway from K. pneumoniae as a synthetic pathway. Moreover, a strain harboring a dual synthetic pathways (ALDH and Pdu) exhibited a 70% increase in 3-HP titer compared to one harboring the ALDH pathway alone (56.1 ± 0.736 mM and 33.1 ± 0.920 mM, respectively). To our knowledge, this is the first report of 3-HP production by E. coli harboring the Pdu pathway, with the dual synthetic pathway showing the highest yield ever reported by batch culture [54.1% (mol/mol)].
3-羟基丙酸（3-HP）是一种重要的C3平台化学品；通过合成途径在重组大肠杆菌中生产3-羟基丙酸一直是众多研究的焦点。当使用甘油作为底物在大肠杆菌中生产3-HP时，仅醛脱氢酶途径（利用醛脱氢酶（ALDH）将3-羟基丙醛（3-HPA）转化为3-HP）被用作合成途径。然而，已知几种细菌（包括肺炎克雷伯菌）能够通过Pdu途径（利用PduP、PduL和PduW酶）生产3-HP。在此，我们报告了以肺炎克雷伯菌的Pdu途径作为合成途径在大肠杆菌中生产3-HP。此外，与仅含有醛脱氢酶途径的菌株相比，含有双合成途径（醛脱氢酶和Pdu）的菌株的3-HP滴度提高了70%（分别为56.1±0.736 mM和33.1±0.920 mM）。据我们所知，这是关于含有Pdu途径的大肠杆菌生产3-HP的首次报道，双合成途径显示出分批培养中报道的最高产量[54.1%（摩尔/摩尔）]。
