Wang Qingqing, Wang Lihong, Han Ruobing, Yang Linying, Zhou Qing, Huang Xiaohua
State Key Laboratory of Food Science and Technology, College of Environment and Civil Engineering, Jiangnan University, Wuxi, China.
Environ Toxicol Chem. 2015 May;34(5):1127-33. doi: 10.1002/etc.2904. Epub 2015 Apr 7.
Bisphenol A (BPA), an emerging pollutant in the environment, has potential toxic effects on plants. The toxicity mechanism, however, remains largely unknown. The antioxidant system plays an important role in protecting plants against the damage of stress. The present study investigated the effects of BPA on the antioxidant system (superoxide dismutase [SOD], peroxidase [POD], catalase [CAT], ascorbic acid [AsA], proline, reduced glutathione [GSH]), reactive oxygen species (ROS; hydrogen peroxide [H2 O2 ], superoxide anion [O2 (-) ]) accumulation, and membrane lipid peroxidation (malondialdehyde [MDA], cell membrane permeability) in soybean seedling roots. The 1.5 mg L(-1) BPA exposure did not affect test indices in the roots. Exposure to 3.0 mg L(-1) , 6.0 mg L(-1) , 12.0 mg L(-1) , or 24.0 mg L(-1) BPA caused increases in SOD (except for 3.0 mg L(-1) BPA) and CAT activities, as well as in AsA, proline, and GSH (except for 3.0 mg L(-1) BPA) content, leading to increases in the H2 O2 and O2 (-) content and to membrane lipid peroxidation. Exposure to 48.0 mg L(-1) or 96.0 mg L(-1) BPA caused decreases in the CAT activity and AsA/GSH content, as well as increases in the SOD and POD activities and the proline content, leading to excess ROS accumulation (i.e., H2 O2 and O2 (-) ) and cell membrane damage. After withdrawal of BPA exposure, ROS accumulation and membrane lipid peroxidation were alleviated by regulating a special antioxidant enzyme or substance.
双酚A（BPA）是环境中一种新出现的污染物，对植物具有潜在毒性作用。然而，其毒性机制在很大程度上仍不清楚。抗氧化系统在保护植物免受胁迫损伤方面发挥着重要作用。本研究调查了双酚A对大豆幼苗根系抗氧化系统（超氧化物歧化酶[SOD]、过氧化物酶[POD]、过氧化氢酶[CAT]、抗坏血酸[AsA]、脯氨酸、还原型谷胱甘肽[GSH]）、活性氧（ROS；过氧化氢[H2O2]、超氧阴离子[O2(-)]）积累以及膜脂过氧化（丙二醛[MDA]、细胞膜通透性）的影响。1.5 mg L(-1)的双酚A暴露对根系的测试指标没有影响。暴露于3.0 mg L(-1)、6.0 mg L(-1)、12.0 mg L(-1)或24.0 mg L(-1)的双酚A会导致SOD（3.0 mg L(-1)双酚A除外）和CAT活性增加，以及AsA、脯氨酸和GSH（3.0 mg L(-1)双酚A除外）含量增加，从而导致H2O2和O2(-)含量增加以及膜脂过氧化。暴露于48.0 mg L(-1)或96.0 mg L(-1)的双酚A会导致CAT活性和AsA/GSH含量降低，以及SOD和POD活性和脯氨酸含量增加，从而导致过量的ROS积累（即H2O2和O2(-)）和细胞膜损伤。在停止双酚A暴露后，通过调节一种特殊的抗氧化酶或物质，ROS积累和膜脂过氧化得到缓解。
