细胞色素和蛋白质在抗坏血酸对含氧气和过氧化氢的人工及微粒体羟基化系统的作用中的贡献。
Contribution of cytochromes and proteins to the effect of ascorbic acid on artificial and microsomal hydroxylation systems containing oxygen and hydrogen peroxide.
作者信息
Chrastil J, Wilson J T
出版信息
Biochem J. 1978 Mar 15;170(3):693-8. doi: 10.1042/bj1700693.
Hydroxylation systems containing cytochromes, proteins and ascorbic acid were studied at physiological pH (7.4) under O2 or N2 with added H2O2. Proteins inhibited aromatic hydroxylation of p-nitrophenol or oxidative demethylation of ethylmorphine in ascorbic acid-containing systems incubated under O2, but strongly activated the systems containing H2O2. Cytochrome c and partially purified cytochrome P-450 from rat liver microsomal preparations activated the system in either O2 or H2O2. The systems needed ascorbic acid (or other enol structures) for activation. Cytochrome iron participated probably in the activation of O2, whereas cytochrome protein participated in a free radical activation of H2O2 (or of O2).
在生理pH值（7.4）下，于氧气或氮气环境中添加过氧化氢，对含有细胞色素、蛋白质和抗坏血酸的羟基化系统进行了研究。在氧气环境下孵育的含抗坏血酸系统中，蛋白质抑制对硝基苯酚的芳香族羟基化或乙基吗啡的氧化脱甲基作用，但对含有过氧化氢的系统有强烈激活作用。细胞色素c和从大鼠肝脏微粒体制剂中部分纯化的细胞色素P - 450在氧气或过氧化氢环境中均可激活该系统。这些系统需要抗坏血酸（或其他烯醇结构）来激活。细胞色素铁可能参与了氧气的激活过程，而细胞色素蛋白参与了过氧化氢（或氧气）的自由基激活过程。
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