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1
Contribution of cytochromes and proteins to the effect of ascorbic acid on artificial and microsomal hydroxylation systems containing oxygen and hydrogen peroxide.细胞色素和蛋白质在抗坏血酸对含氧气和过氧化氢的人工及微粒体羟基化系统的作用中的贡献。
Biochem J. 1978 Mar 15;170(3):693-8. doi: 10.1042/bj1700693.
2
Effect of oxygen concentration on microsomal oxidation of ethanol and generation of oxygen radicals.氧浓度对乙醇微粒体氧化及氧自由基生成的影响。
Biochem J. 1988 May 1;251(3):787-94. doi: 10.1042/bj2510787.
3
[Effect of vitamin B1 deficiency on xenobiotic hydroxylation and lipid peroxidation in rat liver microsomes].
Farmakol Toksikol. 1981 Jan-Feb;44(1):102-4.
4
Primary toxic effects of anthraquinone-2-sulfonic acid in rat liver microsomes.蒽醌 -2-磺酸对大鼠肝微粒体的主要毒性作用。
Toxicol Lett. 1995 Jul;78(2):107-10. doi: 10.1016/0378-4274(94)03238-3.
5
Unusual substituent effects in the hydroxylation of phenols by a Cu(2+)-ascorbic acid-O2 system, gamma-radiolysis, and microsomes.铜（II）-抗坏血酸-O₂体系、γ辐射分解及微粒体对酚类进行羟基化反应时的异常取代基效应
Biochem Biophys Res Commun. 1993 Apr 30;192(2):568-74. doi: 10.1006/bbrc.1993.1453.
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N-Hydroxylation enzymes of carcinogenic aminoazo dyes: possible involvement of cytochrome P-448.致癌性氨基偶氮染料的N-羟基化酶：细胞色素P-448的可能参与
Gan. 1982 Feb;73(1):55-62.
7
The peroxidatic function of liver microsomal cytochrome P-450: comparison of hydrogen peroxide and NADPH-catalysed N-demethylation reactions.肝脏微粒体细胞色素P-450的过氧化物酶功能：过氧化氢与NADPH催化的N-去甲基化反应的比较
Xenobiotica. 1984 Jan-Feb;14(1-2):87-104. doi: 10.3109/00498258409151400.
8
Hydroxylation of p-nitrophenol by rabbit ethanol-inducible cytochrome P-450 isozyme 3a.兔乙醇诱导型细胞色素P-450同工酶3a对对硝基苯酚的羟基化作用。
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9
Protective role of ascorbic acid and alpha-tocopherol on arsenic-induced microsomal dysfunctions.抗坏血酸和α-生育酚对砷诱导的微粒体功能障碍的保护作用。
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[Decrease of cytochrome contents and changes in the kinetics of monooxygenase in liver microsomes of guinea pigs at various stages of L-ascorbic acid].[L-抗坏血酸不同阶段豚鼠肝脏微粒体细胞色素含量降低及单加氧酶动力学变化]
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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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In vitro oxidation of morphine by rat tissue homogenates.吗啡在大鼠组织匀浆中的体外氧化
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The oxidation of drugs by liver microsomes: on the role of TPNH and oxygen.肝脏微粒体对药物的氧化作用：关于三磷酸吡啶核苷酸（TPNH）和氧气的作用
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Ascorbic acid in aromatic hydroxylation. I. A model system for aromatic hydroxylation.抗坏血酸在芳香族羟基化反应中的作用。I. 芳香族羟基化反应的模型体系。
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The colorimetric estimation of formaldehyde by means of the Hantzsch reaction.通过汉茨希反应对比色法测定甲醛。
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6
Cytochrome P-450 as a microsomal peroxidase in steroid hydroperoxide reduction.细胞色素P - 450作为甾体氢过氧化物还原中的微粒体过氧化物酶。
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Ascorbic acid and drug metabolism.抗坏血酸与药物代谢。
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Function of cytochrome P-450 of microsomes.微粒体细胞色素P - 450的功能。
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An affinity column method for partial purification of cytochrome P-450 from phenobarbital-induced rabbit liver microsomes.
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10
Relation of melanoma homogenate and ascorbate solution electron paramagnetic resonance doublets.黑色素瘤匀浆与抗坏血酸溶液电子顺磁共振双峰的关系。
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细胞色素和蛋白质在抗坏血酸对含氧气和过氧化氢的人工及微粒体羟基化系统的作用中的贡献。

Contribution of cytochromes and proteins to the effect of ascorbic acid on artificial and microsomal hydroxylation systems containing oxygen and hydrogen peroxide.

作者信息

Chrastil J, Wilson J T

出版信息

Biochem J. 1978 Mar 15;170(3):693-8. doi: 10.1042/bj1700693.

DOI:10.1042/bj1700693
PMID:25655
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1183948/
Abstract

Hydroxylation systems containing cytochromes, proteins and ascorbic acid were studied at physiological pH (7.4) under O2 or N2 with added H2O2. Proteins inhibited aromatic hydroxylation of p-nitrophenol or oxidative demethylation of ethylmorphine in ascorbic acid-containing systems incubated under O2, but strongly activated the systems containing H2O2. Cytochrome c and partially purified cytochrome P-450 from rat liver microsomal preparations activated the system in either O2 or H2O2. The systems needed ascorbic acid (or other enol structures) for activation. Cytochrome iron participated probably in the activation of O2, whereas cytochrome protein participated in a free radical activation of H2O2 (or of O2).

摘要

在生理pH值（7.4）下，于氧气或氮气环境中添加过氧化氢，对含有细胞色素、蛋白质和抗坏血酸的羟基化系统进行了研究。在氧气环境下孵育的含抗坏血酸系统中，蛋白质抑制对硝基苯酚的芳香族羟基化或乙基吗啡的氧化脱甲基作用，但对含有过氧化氢的系统有强烈激活作用。细胞色素c和从大鼠肝脏微粒体制剂中部分纯化的细胞色素P - 450在氧气或过氧化氢环境中均可激活该系统。这些系统需要抗坏血酸（或其他烯醇结构）来激活。细胞色素铁可能参与了氧气的激活过程，而细胞色素蛋白参与了过氧化氢（或氧气）的自由基激活过程。