Wong P M, Chung S W, Dunbar C E, Bodine D M, Ruscetti S, Nienhuis A W
Clinical Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, Maryland 20892.
Mol Cell Biol. 1989 Feb;9(2):798-808. doi: 10.1128/mcb.9.2.798-808.1989.
A high-titer, recombinant retroviral vector produced in psi 2 packaging cells has been used to introduce the murine interleukin-3 (IL-3) gene into mouse hematopoietic cells. Integration and expression of the IL-3 gene was observed in spleen foci from which could be derived factor-independent, continuously proliferating cell lines. Irradiated or genetically anemic W/Wv recipients of infected hematopoietic cells developed a myeloproliferative syndrome characterized by a marked elevation in leukocyte count, bone marrow hyperplasia, and enlargement of the liver and spleen. The syndrome reflected proliferation of one or more stem cell clones, the progeny of which were capable of repopulating secondary recipients. One animal developed the syndrome primarily by a paracrine mechanism. Endogenous IL-3 production caused amplification of hematopoietic cells but did not appear to alter the maturational or self-renewal potential of these cells.
在psi 2包装细胞中产生的高滴度重组逆转录病毒载体已被用于将小鼠白细胞介素-3（IL-3）基因导入小鼠造血细胞。在脾灶中观察到IL-3基因的整合和表达，从这些脾灶中可以衍生出不依赖因子、持续增殖的细胞系。受感染造血细胞的经照射或基因贫血的W/Wv受体出现了骨髓增殖综合征，其特征为白细胞计数显著升高、骨髓增生以及肝脏和脾脏肿大。该综合征反映了一个或多个干细胞克隆的增殖，其后代能够重新填充二级受体。一只动物主要通过旁分泌机制出现该综合征。内源性IL-3的产生导致造血细胞扩增，但似乎并未改变这些细胞的成熟或自我更新潜能。
