Guiramand J, Nourigat A, Sassetti I, Recasens M
Centre CNRS-INSERM de Pharmacologie-Endrocrinologie, Montpellier, France.
Neurosci Lett. 1989 Mar 27;98(2):222-8. doi: 10.1016/0304-3940(89)90514-4.
K+, excitatory amino acids (EAAs) and carbachol (Carb) were tested separately or in pairs for their ability to stimulate inositol phosphate (IPs) formation in rat forebrain synaptoneurosomes. K+ ions per se, stimulate IPs synthesis (158% of the control value) as well as EAAs and Carb. The glutamate (Glu)- and quisqualate (QA)-elicited IPs formation is not additive with that evoked by K+. Inversely, K+ ions (up to 30 mM) potentiate the Carb-induced IPs accumulation. These results indicate that QA (or Glu) and Carb enhance IPs formation independently and that QA- and K+ -induced IPs responses are interdependent. This suggests that they share a 'common intermediate' step in the multistep mechanism which leads from receptor activation to the IPs synthesis. This 'common intermediate' step may be depolarization and/or Na+ influx.
分别或成对测试钾离子（K⁺）、兴奋性氨基酸（EAAs）和卡巴胆碱（Carb）刺激大鼠前脑突触神经小体中肌醇磷酸（IPs）形成的能力。钾离子本身可刺激IPs合成（为对照值的158%），EAAs和Carb也有此作用。谷氨酸（Glu）和quisqualate（QA）引发的IPs形成与钾离子引发的并非相加关系。相反，钾离子（高达30 mM）可增强Carb诱导的IPs积累。这些结果表明，QA（或Glu）和Carb独立增强IPs形成，且QA和钾离子诱导的IPs反应相互依赖。这表明它们在从受体激活到IPs合成的多步骤机制中共享一个“共同中间”步骤。这个“共同中间”步骤可能是去极化和/或钠离子内流。
