柴油烟尘颗粒催化氧自由基的产生。

Diesel soot particles catalyze the production of oxy-radicals.

作者信息

Vogl G, Elstner E F

机构信息

Institut für Botanik und Mikrobiologie, Technische Universität, Munich, F.R.G.

出版信息

Toxicol Lett. 1989 Apr;47(1):17-23. doi: 10.1016/0378-4274(89)90082-9.

DOI:10.1016/0378-4274(89)90082-9

PMID:2565611

Abstract

The formation of a strong oxidant similar to the OH. radical is catalyzed by diesel soot particles in the presence of cysteine and hydrogen peroxide or in the presence of light. The oxidant(s) formed causes fragmentation of methylthioketobutyric acid measurable as ethylene release. Furthermore, the model carotenoid crocin is bleached and thiobarbituric-acid-reactive material (malondialdehyde) is produced from linolenic acid. All reactions are inhibited by scavengers (propyl gallate, alpha-tocopherol, diazobicyclooctane) and by catalase. The reactions observed suggest that the toxicity and mutagenicity of diesel soot particles is at least in part due to the formation of reactive oxygen species.

摘要

在半胱氨酸和过氧化氢存在下或光照条件下，柴油烟灰颗粒可催化形成类似于羟基自由基的强氧化剂。所形成的氧化剂会导致甲硫基酮丁酸发生碎片化，可通过乙烯释放来测定。此外，模型类胡萝卜素藏红花素会被漂白，亚麻酸会产生硫代巴比妥酸反应性物质（丙二醛）。所有反应均受到清除剂（没食子酸丙酯、α-生育酚、重氮二环辛烷）和过氧化氢酶的抑制。观察到的这些反应表明，柴油烟灰颗粒的毒性和致突变性至少部分归因于活性氧物种的形成。