一种使用带放射性检测的高效液相色谱法检测二氢乳清酸酶的方法。
An assay for dihydroorotase using high-performance liquid chromatography with radioactivity detection.
作者信息
Mehdi S, Wiseman J S
机构信息
Merrell Dow Research Institute, Cincinnati, Ohio 45215.
出版信息
Anal Biochem. 1989 Jan;176(1):105-8. doi: 10.1016/0003-2697(89)90279-0.
PMID:2565694
Abstract
An assay for measuring dihydroorotase activity was devised. Radiolabeled substrate and product were separated by high-performance liquid chromatography using a reverse-phase column with ion-pairing, and the radioactivity was quantitated by flow detection.
摘要
设计了一种用于测量二氢乳清酸酶活性的检测方法。使用带有离子对的反相柱，通过高效液相色谱法分离放射性标记的底物和产物，并通过流动检测对放射性进行定量。
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