低水平的Bax抑制因子-1基因表达会增加衣霉素诱导的人神经母细胞瘤SY5Y细胞凋亡。

Wu Dan, Wang Peirong, Wang Shiyao

Department of Medical Genetics, School of Basic Medical Sciences, Peking University, Beijing 100191, China.

Department of Medical Genetics, School of Basic Medical Sciences, Peking University, Beijing 100191, China ; Institute of Biophysics, Chinese Academy of Sciences, Beijing 100190, China.

Neural Regen Res. 2012 Jun 15;7(17):1331-7. doi: 10.3969/j.issn.1673-5374.2012.17.008.

A human SH-SY5Y neuroblastoma cell line with a low level of Bax inhibitor-1 expression was established by lentivirus-mediated RNA interference and fluorescence-activated cell sorting. In control SH-SY5Y cells, tunicamycin treatment induced endoplasmic reticulum stress-mediated apoptosis; however, after Bax inhibitor-1 gene knockdown, cell survival rates were significantly decreased and the degree of apoptosis was significantly increased following tunicamycin treatment. In addition, chromatin condensation and apparent apoptotic phenomena, such as marginalization and cytoplasmic vesicles, were observed. Our findings indicate that Bax inhibitor-1 can delay apoptosis induced by endoplasmic reticulum stress.

通过慢病毒介导的RNA干扰和荧光激活细胞分选，建立了一种低水平表达Bax抑制因子-1的人SH-SY5Y神经母细胞瘤细胞系。在对照SH-SY5Y细胞中，衣霉素处理诱导内质网应激介导的细胞凋亡；然而，在敲低Bax抑制因子-1基因后，衣霉素处理后细胞存活率显著降低，凋亡程度显著增加。此外，还观察到染色质浓缩以及明显的凋亡现象，如边缘化和细胞质小泡。我们的研究结果表明，Bax抑制因子-1可以延迟内质网应激诱导的细胞凋亡。