Parmentier M, De Vijlder J J, Muir E, Szpirer C, Islam M Q, Geurts van Kessel A, Lawson D E, Vassart G
IRIBHN, Medical School, Free University of Brussels, Belgium.
Genomics. 1989 Apr;4(3):309-19. doi: 10.1016/0888-7543(89)90335-2.
The 5' and 3' regions of the human gene coding for calbindin 27 kDa were cloned and sequenced. Structural features of the 5' region included the presence of an Alu repeat and two elements regularly associated with eukaryotic promoters: an alternating purine-pyrimidine element and a homopurine-homopyrimidine box. The 3' region contained a second Alu family member and a degenerate 1.4-kb L1 repeat. A comparison with the chicken promoter was made in order to define regions conserved in evolution and potentially important in gene expression regulation. The greater similarity is located around the TATA box, but strongly conserved elements were not found. The gene was assigned to chromosome 8 by using human-rodent hybrid cell lines. Two restriction fragment length polymorphisms (HindIII and SacI) were detected with a cDNA probe recognizing the 3' end of the gene.
对编码27kDa钙结合蛋白的人类基因的5'和3'区域进行了克隆和测序。5'区域的结构特征包括存在一个Alu重复序列以及两个与真核启动子经常相关的元件：一个嘌呤 - 嘧啶交替元件和一个同嘌呤 - 同嘧啶盒。3'区域包含第二个Alu家族成员和一个退化的1.4kb L1重复序列。为了确定在进化中保守且可能在基因表达调控中重要的区域，对鸡的启动子进行了比较。更大的相似性位于TATA盒周围，但未发现强保守元件。通过使用人 - 啮齿动物杂交细胞系将该基因定位到8号染色体上。用识别该基因3'末端的cDNA探针检测到两个限制性片段长度多态性（HindIII和SacI）。
