Jiang Minjie, Wang Lisheng, Liu Xu, Yang Hua, Ren Fan, Gan Lizhen, Jiang Weizhe
School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, China.
School of Pharmaceutical, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, China.
Int J Mol Sci. 2015 Feb 4;16(2):3441-51. doi: 10.3390/ijms16023441.
A temperature-sensitive matrine-imprinted polymer was prepared in chloroform by free-radical cross-linking copolymerization of methacrylic acid at 60 °C in the presence of ethylene glycol dimethacrylate as the cross-linker, N-isopropyl acrylamide as the temperature-responsive monomer and matrine as the template molecule. Binding experiments and Scatchard analyses revealed that two classes of binding sites were formed on molecular imprinted polymer (MIP) at 50 °C. Additionally, the thermoresponsive MIP was tested for its application as a sorbent material for the selective separation of matrine from Chinese medicinal plant radix Sophorae tonkinensis. It was shown that the thermoresponsive MIP displayed different efficiency in clean-up and enrichments using the SPE protocol at different temperatures.
通过在60℃下，以乙二醇二甲基丙烯酸酯作为交联剂、N-异丙基丙烯酰胺作为温度响应性单体、苦参碱作为模板分子，在氯仿中进行甲基丙烯酸的自由基交联共聚反应，制备了一种温度敏感型苦参碱印迹聚合物。结合实验和Scatchard分析表明，在50℃时分子印迹聚合物（MIP）上形成了两类结合位点。此外，还测试了热响应性MIP作为吸附剂材料从中药广豆根中选择性分离苦参碱的应用。结果表明，热响应性MIP在不同温度下使用固相萃取方案进行净化和富集时表现出不同的效率。
