在哺乳动物DNA复制起始过程中，MCM复合蛋白与核基质的瞬时关联。
Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication.
作者信息
Hesketh Emma L, Knight John R P, Wilson Rosemary H C, Chong James P J, Coverley Dawn
机构信息
a Department of Biology ; University of York ; York , UK.
出版信息
Cell Cycle. 2015;14(3):333-41. doi: 10.4161/15384101.2014.980647.
The minichromosome maintenance complex (MCM2-7) is the putative DNA helicase in eukaryotes, and essential for DNA replication. By applying serial extractions to mammalian cells synchronized by release from quiescence, we reveal dynamic changes to the sub-nuclear compartmentalization of MCM2 as cells pass through late G1 and early S phase, identifying a brief window when MCM2 becomes transiently attached to the nuclear-matrix. The data distinguish 3 states that correspond to loose association with chromatin prior to DNA replication, transient highly stable binding to the nuclear-matrix coincident with initiation, and a post-initiation phase when MCM2 remains tightly associated with chromatin but not the nuclear-matrix. The data suggests that functional MCM complex loading takes place at the nuclear-matrix.
微小染色体维持复合物（MCM2 - 7）是真核生物中假定的DNA解旋酶，对DNA复制至关重要。通过对从静止状态释放后同步化的哺乳动物细胞进行连续提取，我们揭示了随着细胞通过G1晚期和S期早期，MCM2亚核区室化的动态变化，确定了MCM2短暂附着于核基质的一个短暂窗口。数据区分了3种状态，分别对应于DNA复制前与染色质的松散结合、与起始同步的与核基质的短暂高度稳定结合，以及起始后阶段，此时MCM2仍与染色质紧密结合但不与核基质结合。数据表明功能性MCM复合物加载发生在核基质处。
Zotero 插件