移植到W/Wv和Sl/Sld基因型生殖细胞缺陷小鼠睾丸中的生精小管内生殖细胞的分化。

Differentiation of germ cells in seminiferous tubules transplanted to testes of germ cell-deficient mice of W/Wv and Sl/Sld genotypes.

作者信息

Kuroda H, Nakayama H, Namiki M, Matsumoto K, Nishimune Y, Kitamura Y

机构信息

Department of Pathology, Osaka University Medical School, Japan.

出版信息

J Cell Physiol. 1989 May;139(2):329-34. doi: 10.1002/jcp.1041390215.

DOI:10.1002/jcp.1041390215

PMID:2565907

Abstract

(WB X C57BL/6)F1-W/Wv (hereafter, WBB6F1-W/Wv) mice and (WC X C57BL/6)F1-Sl/Sld (hereafter, WCB6F1-Sl/Sld) mice are sterile due to the deficient spermatogenesis in the testes. The cause of deficient spermatogenesis in WBB6F1-W/Wv mice is considered to be a defect in germ cells themselves, whereas that in WCB6F1-Sl/Sld mice is considered to be a defect in tissue environment necessary for differentiation of germ cells. Seminiferous tubules isolated from cryptorchid testes of C57BL/6- +/+ mice were transplanted into the testes of WBB6F1-W/Wv and WCB6F1-Sl/Sld mice to clarify that the extratubular environment of these mice was intact or not. Type A spermatogonia in the transplanted tubules normally differentiated into spermatids, suggesting that the extratubular environment is intact in both WBB6F1-W/Wv and WCB6F1-Sl/Sld mice.

摘要

（WB×C57BL/6）F1-W/Wv小鼠（以下简称WBB6F1-W/Wv小鼠）和（WC×C57BL/6）F1-Sl/Sld小鼠（以下简称WCB6F1-Sl/Sld小鼠）因睾丸生精功能缺陷而不育。WBB6F1-W/Wv小鼠生精功能缺陷的原因被认为是生殖细胞本身存在缺陷，而WCB6F1-Sl/Sld小鼠生精功能缺陷的原因被认为是生殖细胞分化所需的组织环境存在缺陷。将从C57BL/6- +/+小鼠隐睾中分离出的生精小管移植到WBB6F1-W/Wv和WCB6F1-Sl/Sld小鼠的睾丸中，以阐明这些小鼠的管外环境是否完整。移植小管中的A型精原细胞正常分化为精子细胞，这表明WBB6F1-W/Wv和WCB6F1-Sl/Sld小鼠的管外环境都是完整的。