Jiang Yong, Wang Hui-Yan, Zheng Sheng, Mu Shang-Qiang, Ma Meng-Ni, Xie Xin, Zhang Yang-Yang, Zhang Chun-Xue, Cai Jian-Hui
Laboratory Medical College, Jilin Medical College, Jilin, PR China.
Laboratory Medical College, Jilin Medical College, Jilin, PR China.
Cardiovasc Pathol. 2015 Jul-Aug;24(4):224-9. doi: 10.1016/j.carpath.2014.12.003. Epub 2014 Dec 27.
Hypertension is the most common risk factor for various cardiovascular and cerebrovascular diseases that affects approximately 61 million, or 25% of the population in United States. The dietary salt intake is one of the most important but modifiable factors for hypertension. In the current study, we aim to elucidate the role of aquaporin 1 in high-salt-induced hypertension and cardiac injuries and whether angiotensin II receptor blocker valsartan could ameliorate the effect of high salt on blood pressure. Mice were fed with normal diet, high-salt diet in the presence or absence of valsartan for 4 weeks. The body weight gain, feeding behavior, blood pressure, and cardiac pathology changes were monitored after 4 weeks. The expression of aquaporin 1, vascular endothelial growth factor, transforming growth factor β1, and basic fibroblast growth factor were analyzed using quantitative real-time polymerase chain reaction, Western blot, and immunohistochemical staining. Valsartan partially reversed the effects of high-salt diet on hypertension, cardiac injuries such as fibrosis and inflammatory cell infiltration, and inhibition of aquaporin 1 and angiogenic factors; valsartan alone did not exert such effects. The current data demonstrated that the reduction of cardiac aquaporin 1 and angiogenic factor expression level might be associated with high-salt-induced hypertension and cardiac injuries in mice, which could be ameliorated by angiotensin II receptor blocker treatment.
高血压是各种心脑血管疾病最常见的危险因素，在美国约有6100万人受其影响，占总人口的25%。饮食中的盐摄入量是高血压最重要但可改变的因素之一。在本研究中，我们旨在阐明水通道蛋白1在高盐诱导的高血压和心脏损伤中的作用，以及血管紧张素II受体阻滞剂缬沙坦是否能改善高盐对血压的影响。将小鼠分为正常饮食组、高盐饮食组、高盐饮食加缬沙坦组，喂养4周。4周后监测小鼠体重增加、摄食行为、血压和心脏病理变化。采用定量实时聚合酶链反应、蛋白质免疫印迹和免疫组织化学染色分析水通道蛋白1、血管内皮生长因子、转化生长因子β1和碱性成纤维细胞生长因子的表达。缬沙坦部分逆转了高盐饮食对高血压、心脏损伤（如纤维化和炎性细胞浸润）以及水通道蛋白1和血管生成因子抑制的影响；单独使用缬沙坦则没有这种效果。目前的数据表明，小鼠心脏水通道蛋白1和血管生成因子表达水平的降低可能与高盐诱导的高血压和心脏损伤有关，而血管紧张素II受体阻滞剂治疗可以改善这种情况。
