甘油二缩水甘油醚交联氧化淀粉微凝胶中溶菌酶的控制摄取和释放。
Controlled uptake and release of lysozyme from glycerol diglycidyl ether cross-linked oxidized starch microgel.
机构信息
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, College of Life Science and Technology, P. O. Box 53, 100029 Beijing, China.
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, College of Life Science and Technology, P. O. Box 53, 100029 Beijing, China.
出版信息
Carbohydr Polym. 2015 May 5;121:276-83. doi: 10.1016/j.carbpol.2015.01.002. Epub 2015 Jan 7.
A biodegradable microgel system based on glycerol-1,3-diglycidyl ether (GDGE) cross-linked TEMPO-oxidized potato starch polymers was developed for controlled uptake and release of proteins. A series of microgels were prepared with a wide range of charge density and cross-link density. We found both swelling capacity (SWw) and lysozyme uptake at saturation (Γsat) increased with increasing degree of oxidation (DO) and decreasing cross-link density. Microgel of DO100% with a low cross-link density (RGDGE/polymer (w/w) of 0.025) was selected to be the optimum gel type for lysozyme absorption; Γsat increased with increasing pH and decreasing ionic strength. It suggests that the binding strength was the strongest at high pH and low ionic strength, which was recognized as the optimum absorption conditions. The lysozyme release was promoted at low pH and high ionic strength, which were considered to be the most suitable conditions for triggering protein release. These results may provide useful information for the controlled uptake and release of proteins by oxidized starch microgels.
基于甘油-1,3-二缩水甘油醚（GDGE）交联的 TEMPO 氧化马铃薯淀粉聚合物的可生物降解微凝胶系统被开发用于控制蛋白质的摄取和释放。用一系列具有广泛电荷密度和交联密度的微凝胶进行制备。我们发现，随着氧化度（DO）的增加和交联密度的降低，溶胀能力（SWw）和溶菌酶饱和摄取量（Γsat）都增加。选择 DO100%、交联密度低（RGDGE/聚合物（w/w）为 0.025）的微凝胶作为溶菌酶吸收的最佳凝胶类型；Γsat随 pH 值的增加和离子强度的降低而增加。这表明在高 pH 值和低离子强度下结合强度最强，这被认为是最佳的吸收条件。在低 pH 值和高离子强度下，溶菌酶的释放得到促进，这被认为是触发蛋白质释放的最适合条件。这些结果可能为氧化淀粉微凝胶对蛋白质的控制摄取和释放提供有用的信息。
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