谷胱甘肽包覆的碲化镉纳米晶体作为测定芬苯达唑的探针。

Glutathione-capped CdTe nanocrystals as probe for the determination of fenbendazole.

作者信息

Li Qin, Tan Xuanping, Li Jin, Pan Li, Liu Xiaorong

机构信息

Chongqing Medical and Health School, Fuling, Chongqing 408100, PR China.

School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2015 Apr 15;141:10-5. doi: 10.1016/j.saa.2015.01.026. Epub 2015 Jan 23.

DOI:10.1016/j.saa.2015.01.026

PMID:25659737

Abstract

Water-soluble glutathione (GSH)-capped CdTe quantum dots (QDs) were synthesized. In pH 7.1 PBS buffer solution, the interaction between GSH-capped CdTe QDs and fenbendazole (FBZ) was investigated by spectroscopic methods, including fluorescence spectroscopy, ultraviolet-visible absorption spectroscopy, and resonance Rayleigh scattering (RRS) spectroscopy. In GSH-capped CdTe QDs solution, the addition of FBZ results in the fluorescence quenching and RRS enhancement of GSH-capped CdTe QDs. And the quenching intensity (enhanced RRS intensity) was proportional to the concentration of FBZ in a certain range. Investigation of the interaction mechanism, proved that the fluorescence quenching and RRS enhancement of GSH-capped CdTe QDs by FBZ is the result of electrostatic attraction. Based on the quenching of fluorescence (enhancement of RRS) of GSH-capped CdTe QDs by FBZ, a novel, simple, rapid and specific method for FBZ determination was proposed. The detection limit for FBZ was 42 ng mL(-1) (3.4 ng mL(-1)) and the quantitative determination range was 0-2.8 μg mL(-1) with a correlation of 0.9985 (0.9979). The method has been applied to detect FBZ in real simples and with satisfactory results.

摘要

合成了水溶性谷胱甘肽（GSH）包覆的碲化镉量子点（QDs）。在pH 7.1的磷酸盐缓冲溶液（PBS）中，采用荧光光谱、紫外可见吸收光谱和共振瑞利散射（RRS）光谱等光谱方法研究了GSH包覆的CdTe量子点与芬苯达唑（FBZ）之间的相互作用。在GSH包覆的CdTe量子点溶液中，加入FBZ会导致GSH包覆的CdTe量子点的荧光猝灭和RRS增强。并且猝灭强度（增强后的RRS强度）在一定范围内与FBZ的浓度成正比。对相互作用机理的研究表明，FBZ对GSH包覆的CdTe量子点的荧光猝灭和RRS增强是静电吸引的结果。基于FBZ对GSH包覆的CdTe量子点荧光的猝灭（RRS的增强），提出了一种新颖、简单、快速且特异的FBZ测定方法。FBZ的检测限为42 ng mL⁻¹（3.4 ng mL⁻¹），定量测定范围为0 - 2.8 μg mL⁻¹，相关系数为0.9985（0.9979）。该方法已应用于实际样品中FBZ的检测，结果令人满意。