Santoro Domenico, Ahrens Kim, Marsella Rosanna, Segre Mariangela
Department of Small Animal Clinical Sciences, University of Florida, Gainesville, FL, USA; Department of Dermatology, College of Medicine, University of Florida, Gainesville, FL, USA.
Exp Dermatol. 2015 Apr;24(4):317-9. doi: 10.1111/exd.12660.
Increased secretion of antimicrobial peptides and cytokines is present in atopic skin. The purpose of this study was to compare the production of β-defensin (cBD)3-like, cathelicidin (cCath) and cytokines in atopic and healthy canine keratinocytes. Seven atopic house dust mites (HDMs) sensitive and five healthy age-matched beagles were used. Keratinocytes were stimulated for 24 h, and the supernatant was collected. A significantly higher production of cBD3-like was present at baseline in atopic compared with healthy keratinocytes, but cBD3-like did not increase after stimulation. IL-17 and lipopolysaccharide increased cBD3-like in healthy compared with atopic keratinocytes. cCath increased in both groups after stimulation. Atopic keratinocytes exposed to HDM produced more IL-8 and keratinocyte-derived chemokine-like than healthy keratinocytes. Exposure to HDM induced an increased IL-8 in atopic keratinocytes and a decreased IFN-γ in healthy keratinocytes. These results may suggest an over sensitization of atopic keratinocytes and a possible impairment of the cutaneous defense against micro-organisms.
特应性皮肤中抗菌肽和细胞因子的分泌增加。本研究的目的是比较特应性犬和健康犬角质形成细胞中β-防御素（cBD）3样、cathelicidin（cCath）和细胞因子的产生情况。使用了7只对屋尘螨（HDM）敏感的特应性犬和5只年龄匹配的健康比格犬。角质形成细胞被刺激24小时，然后收集上清液。与健康角质形成细胞相比，特应性角质形成细胞在基线时cBD3样的产生显著更高，但刺激后cBD3样没有增加。与特应性角质形成细胞相比，IL-17和脂多糖使健康角质形成细胞中的cBD3样增加。两组在刺激后cCath均增加。暴露于HDM的特应性角质形成细胞比健康角质形成细胞产生更多的IL-8和角质形成细胞衍生趋化因子样物质。暴露于HDM导致特应性角质形成细胞中IL-8增加，健康角质形成细胞中IFN-γ减少。这些结果可能表明特应性角质形成细胞过度敏感，以及皮肤对微生物防御可能受损。
