Lei L, Gang S, Lu W Y
Department of Physiology, Shandong Medical University, Jinan, Shandong, The People's Republic of China.
Respir Physiol. 1989 Jan;75(1):65-73. doi: 10.1016/0034-5687(89)90087-x.
The responses in phrenic nerve activity resulting from stimulation and successive focal block of the dorso-medial area of nucleus facialis (dmNF) were studied in urethane anaesthetized rabbits. It was found that: (1) Long train stimulation (0.025-0.2 mA, 100 Hz) delivered to the dmNF caused marked prolongation in inspiratory duration and distinct increase in amplitude of phrenic discharge. Continuous inspiration with increased amplitude was usually observed during stimulation. (2) Short train stimulation (10 pulses, 100 Hz, 0.025-0.2 mA) during inspiration increased the duration and amplitude of inspiration. When the short train stimulation was delivered during expiration, inspiration would be initiated prematurely. (3) Microinjection of L-glutamate (0.5 M, 0.5-2 microliters) into the dmNF also increased inspiratory duration and amplitude. (4) Focal block of the dmNF by microinjection of lidocaine (2%, 1-2 microliters) produced strong depression or complete cessation of inspiration. In conclusion, the results of the present study show that the dmNF may play an important role in the neurogenesis of respiratory rhythm.
在氨基甲酸乙酯麻醉的家兔中，研究了面神经背内侧区（dmNF）受到刺激和相继局部阻滞时膈神经活动的反应。结果发现：（1）给予dmNF长时间串刺激（0.025 - 0.2 mA，100 Hz）可使吸气持续时间显著延长，膈神经放电幅度明显增加。刺激期间通常观察到吸气幅度增加的持续性吸气。（2）吸气期间给予短串刺激（10个脉冲，100 Hz，0.025 - 0.2 mA）可增加吸气的持续时间和幅度。当在呼气期间给予短串刺激时，吸气会提前启动。（3）向dmNF微量注射L - 谷氨酸（0.5 M，0.5 - 2微升）也可增加吸气持续时间和幅度。（4）通过微量注射利多卡因（2%，1 - 2微升）对dmNF进行局部阻滞可导致吸气强烈抑制或完全停止。总之，本研究结果表明，dmNF可能在呼吸节律的神经发生中起重要作用。
