Gomis R, Casanovas A, Malaisse W J
Endocrinology and Diabetes Unit, Hospital Clinic, Barcelona University, Spain.
Biosci Rep. 1989 Feb;9(1):55-61. doi: 10.1007/BF01117511.
Rat pancreatic islet homogenates catalyze the incorporation of [2,5-3H]histamine into endogenous proteins recovered in both the stacking gel and a Mr 84000 protein separated by polyacrylamide electrophoresis. The labelling of these proteins represents a Ca2+-dependent process inhibited by glycine methylester, but not sarcosine methylester, and enhanced after preincubation of the islets at a high concentration of D-glucose. Although transglutaminase activity is found in both soluble and particulate subcellular fractions, the endogenous transglutaminase substrates were located mainly in particulate, possibly membrane-associated, material.
