前强啡肽原基因腺病毒载体递送对大鼠福尔马林诱导的实验性炎性疼痛的影响。
Effect of adenoviral delivery of prodynorphin gene on experimental inflammatory pain induced by formalin in rats.
作者信息
Chen Xionggang, Wang Tingting, Lin Caizhu, Chen Baihong
机构信息
Department of Anesthesiology, The First Affiliated Hospital of Fujian Medical University Fuzhou 350005, P. R. China.
Department of Ophthalmology, The First Affiliated Hospital of Fujian Medical University Fuzhou 350005, P. R. China.
出版信息
Int J Clin Exp Med. 2014 Dec 15;7(12):4877-86. eCollection 2014.
Circumstantial evidences suggest that dynorphins and their common precursor prodynorphin (PDYN) are involved in antinociception and neuroendocrine signaling. DREAM knockout mice had increased levels of PDYN and dynorphin expression, and reduced sensitivity to painful stimuli. However, some data support the notion that the up-regulation of spinal dynorphin expression is a common critical feature in neuropathic pain. It is not clear whether the production of dynorphin A can be increased when more PDYN is present. In this study we investigated the changes in pain behaviors, spinal PDYN mRNA expression and dynorphin A production on formalin-induced pain in rats receiving the pretreatment of adenoviral delivery of PDYN. Our results showed that the adenoviral transfer of PDYN gene was sufficient to reduce pain behaviors resulting from formalin injection, and the antinociceptive effect after receiving the pretreatment of adenoviral delivery of PDYN was mediated at the level of the spinal cord via KOR.
间接证据表明强啡肽及其共同前体前强啡肽原（PDYN）参与了抗伤害感受和神经内分泌信号传导。DREAM基因敲除小鼠的PDYN和强啡肽表达水平升高，对疼痛刺激的敏感性降低。然而，一些数据支持脊髓强啡肽表达上调是神经性疼痛的一个常见关键特征这一观点。当存在更多PDYN时，强啡肽A的产生是否会增加尚不清楚。在本研究中，我们调查了接受PDYN腺病毒递送预处理的大鼠在福尔马林诱导的疼痛中疼痛行为、脊髓PDYN mRNA表达和强啡肽A产生的变化。我们的结果表明，PDYN基因的腺病毒转移足以减轻福尔马林注射引起的疼痛行为，并且在接受PDYN腺病毒递送预处理后的抗伤害感受作用是通过脊髓水平的κ阿片受体（KOR）介导的。
Zotero 插件