Synthetic egg-laying hormone of Aplysia: quantitative studies of induction of egg laying in Stylocheilus and of the activation of Aplysia buccal neuron B16.
作者信息
Ram J L, Ram M L
机构信息
Department of Physiology, Wayne State University, Detroit, MI 48201.
出版信息
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1989;92(1):131-4. doi: 10.1016/0742-8413(89)90214-4.
Synthetic Aplysia egg-laying hormone (ELH-lysine-amide) elicited egg laying in Stylocheilus at a threshold dose of 0.5 microgram per recipient, estimated to be a concentration in the circulation of Stylocheilus of approximately 70 nM. 2. Threshold level and size of egg mass produced by ELH-lysine-amide and bag cell extracts (containing biological ELH) were not significantly different. Latency to lay of recipients of 0.5-4.0 micrograms ELH-lysine-amide (30 +/- 1 min) was significantly longer (P less than 0.05) than for bag cell extract recipients (21 +/- 1 min). 3. ELH-lysine-amide depolarized and activated action potentials in Aplysia buccal neuron B16 in high magnesium, low calcium medium. 4. The lowest concentration of ELH-lysine-amide to activate a supra-threshold response of left and right B16 neurons ranged from 250 nM to 1 microM. 5. Threshold levels for responses to synthetic ELH-lysine-amide and to biological ELH were approximately the same in both egg-laying assay and electrophysiological assay, indicating the likely identity of synthetic and biological ELH. However, the shorter egg-laying latency with bag cell extract suggests that there may be additional factors in the extract that facilitate egg laying.
