Imamura Daisuke, Mizuno Tamaki, Miyoshi Shin-ichi, Shinoda Sumio
Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases, First Floor ID Hospital Campus, 57 Dr. S. C. Banerjee Road, Beliaghata, Kolkata, 700010, India.
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Kita-ku, Okayama, 700-8530, Japan.
Microbiol Immunol. 2015 May;59(5):305-10. doi: 10.1111/1348-0421.12246.
Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.
许多细菌种类在不适合生长的条件下会进入活的非可培养（VBNC）状态。在本研究中，发现VBNC状态的霍乱弧菌细胞复苏的条件会随时间变化。虽然最初VBNC细胞可通过过氧化氢酶或HT - 29细胞提取物处理转化为可培养状态，但随后它们进入了一种不能被这些因素转化为可培养状态的情形。然而，荧光显微镜显示此状态下存在活细胞，通过与HT - 29人结肠腺癌细胞共培养可使VBNC细胞复苏。最终，所有细胞都进入了即使与HT - 29共培养也无法复苏的状态。VBNC状态细胞的这些特征性变化是霍乱弧菌O1和O139血清群菌株的共同特征。因此，霍乱弧菌的VBNC状态并非单一特性，而是随时间持续变化。
