Diwaker Drishya, Mishra Kamla P, Ganju Lilly, Singh Shashi B
Immunomodulation Laboratory, Defence Institute of Physiology and Allied Sciences , Delhi, India .
Viral Immunol. 2015 Apr;28(3):153-60. doi: 10.1089/vim.2014.0095. Epub 2015 Feb 9.
Dengue virus (DENV) causes a febrile disease, infecting around 50-100 million people annually. The relationship between DENV proteins and host cellular responses during infection is unclear. This study investigated the interaction of host protein disulfide isomerase (PDI) with DENV proteins and role of lipid rafts in viral immunopathogenesis. Host viral protein interactions were studied by co-immunoprecipitation and co-localization. It was found that PDI interacts with DENV nonstructural protein 1 (NS1) intracellularly as well as on the surface in the lipid raft domain. Disruption of this key interaction between PDI and NS1 could be an important therapeutic strategy to block DENV infection.
登革病毒（DENV）引发一种发热性疾病，每年感染约5000万至1亿人。感染期间DENV蛋白与宿主细胞反应之间的关系尚不清楚。本研究调查了宿主蛋白二硫键异构酶（PDI）与DENV蛋白的相互作用以及脂筏在病毒免疫发病机制中的作用。通过免疫共沉淀和共定位研究宿主病毒蛋白相互作用。结果发现，PDI在细胞内以及脂筏结构域的表面与DENV非结构蛋白1（NS1）相互作用。破坏PDI与NS1之间的这种关键相互作用可能是阻断DENV感染的一种重要治疗策略。
