Hartikainen Hanna, Filippenko Dmitry, Okamura Beth, Vasemägi Anti
Department of Life Sciences, Natural History Museum, London SW7 5BD, UK.
Dis Aquat Organ. 2015 Feb 10;113(1):85-8. doi: 10.3354/dao02833.
Proliferative kidney disease (PKD) caused by the myxozoan parasite Tetracapsuloides bryosalmonae is a severe parasitic disease of salmonid fish. Estimates of genetic variation in parasite populations across Europe are currently lacking. We developed the first polymorphic microsatellite markers for T. bryosalmonae using Illumina MiSeq sequence data derived from genomic DNA. Twelve polymorphic loci were identified from 24 tested loci. Allelic variation was low at most loci, ranging from 2 to 6 (average 3.0). The markers developed here are expected to be useful in future genetic studies of T. bryosalmonae, aimed at further understanding the dispersal of the parasite, host-parasite relationships and the epidemiology of PKD.
由粘孢子虫寄生虫脑粘体虫（Tetracapsuloides bryosalmonae）引起的增殖性肾病（PKD）是鲑科鱼类的一种严重寄生虫病。目前尚缺乏对欧洲各地寄生虫种群遗传变异的估计。我们利用从基因组DNA中获得的Illumina MiSeq序列数据，开发了首个针对脑粘体虫的多态性微卫星标记。从24个测试位点中鉴定出12个多态性位点。大多数位点的等位基因变异较低，范围为2至6（平均3.0）。预计这里开发的标记将在未来脑粘体虫的遗传研究中发挥作用，旨在进一步了解寄生虫的传播、宿主 - 寄生虫关系以及增殖性肾病的流行病学。
