[Erythropoietin influence on the blood oxygen transport and prooxidant-antioxidant state during hepatic ischemia-reperfusion].

The parameters of blood oxygen transport, the products of lipid peroxidation and antioxidative factors were determined in rats during the hepatic ischemia/reperfusion (HIR) under preliminary single injections of different doses ofrecombinant human erythropoietin (rhEPO). Hepatic ischemia was induced for 30 min by Pringle maneuver, reperfusion lasted 120 min. The experiments had shown that HIR led to the significant decreases in the hemoglobin oxygen affinity, activation of lipid peroxidation, depletion of the antioxidant system, increases blood transaminases (ALT and AST). rhEPO in dose 100 IU/kg aggravates decreasing hemoglobin oxygen affinity, improves some antioxidant parameters, but didn't correct lipid peroxidation or plasma transaminases during HIR. rhEPO infusion id dose 1000 IU/kg leads to oxyhemoglobin dissociative curve shift left-wards, improves prooxidant-antioxidant balance and plasma ALT, AST activities at the end of re-perfusion period.