Saïdi S A, Abdelkafi S, Jbahi S, van Pelt J, El-Feki A
Liver Research Facility/Labo Hepatology, University Hospital Gasthuisberg, Leuven, Belgium Laboratory of Animal Ecophysiology, Faculty of Sciences, University of Sfax, Sfax, Tunisia
Département de Génie Biologique, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, Sfax, Tunisia.
Hum Exp Toxicol. 2015 Mar;34(3):249-59. doi: 10.1177/0960327114531991. Epub 2014 Jun 16.
This study investigated the hypothesis that administration of tilapia fish oil diet would attenuate warm liver ischemia/reperfusion injury (IRI) and whether fish oil modulates prooxidant/antioxidant status. Male Wistar rats were subjected to 30 min of approximately 70% hepatic ischemia followed by 1, 12, and 24 h reperfusion. Rats were randomly divided into three groups: sham-operated group (SO), control-warm hepatic ischemia (WI) group, and Oil-WI group given tilapia oil for 3 weeks followed by liver IRI. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were measured in the plasma. Levels of thiobarbituric acid reactive substances (TBARS) and antioxidant enzymes as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities were measured in liver fractions. In the sham group, there was no enzymatic or histological change. I/R caused significant increase in serum AST, ALT, and tissue TBARS levels. As compared to the control group, animals treated with tilapia oil experienced a significant decrease (p < 0.05) in AST and ALT levels in reperfusion periods. Tissue TBARS levels in Oil-WI group were significantly (p < 0.05) reduced as compared to control group at 60 min after reperfusion. After ischemia, 1, 12, and 24 h of reperfusion, CAT, SOD, and GPx values were the lowest in the Oil-WI group and highest in the control group and were statistically significant (p < 0.05). Histological analysis also revealed that fish oil provided some protection compared with the control group. Tilapia oil exerts a protective effect during the early phase of reperfusion, and it modulates prooxidant/antioxidant status of rat liver subjected to warm IRI.
本研究调查了罗非鱼鱼油饮食给药可减轻肝脏热缺血/再灌注损伤（IRI）这一假说，以及鱼油是否调节促氧化剂/抗氧化剂状态。雄性Wistar大鼠经历约70%肝脏缺血30分钟，随后再灌注1、12和24小时。大鼠随机分为三组：假手术组（SO）、对照-肝脏热缺血（WI）组和Oil-WI组，后者给予罗非鱼油3周后进行肝脏IRI。测定血浆中天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平。测定肝脏组分中硫代巴比妥酸反应性物质（TBARS）水平以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）等抗氧化酶活性。在假手术组中，无酶学或组织学变化。缺血/再灌注导致血清AST、ALT和组织TBARS水平显著升高。与对照组相比，用罗非鱼油处理的动物在再灌注期AST和ALT水平显著降低（p<0.05）。再灌注60分钟后，Oil-WI组的组织TBARS水平与对照组相比显著降低（p<0.05）。缺血后再灌注1、12和24小时，Oil-WI组的CAT、SOD和GPx值最低，对照组最高，且具有统计学意义（p<0.05）。组织学分析还显示，与对照组相比，鱼油提供了一定保护。罗非鱼油在再灌注早期发挥保护作用，并调节热IRI大鼠肝脏的促氧化剂/抗氧化剂状态。
