Abdel-Rahman Mohamed Ali, Tashiro Yukihiro, Zendo Takeshi, Sakai Kenji, Sonomoto Kenji
Laboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan Botany and Microbiology Department, Faculty of Science (Boys branch), Al-Azhar University, PN:11884, Nasr City, Cairo, Egypt.
Laboratory of Soil Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Japan, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan Institute of Advanced Study, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
FEMS Microbiol Lett. 2015 Jan;362(2):1-7. doi: 10.1093/femsle/fnu030. Epub 2014 Dec 4.
Production of optically pure lactic acid from lignocellulosic material for commercial purposes is hampered by several difficulties, including heterofermentation of pentose sugars and high energy consumption by mesophilic lactic acid bacteria. Here, we report a novel lactic acid bacterium, strain QU 50, that has the potential to produce optically pure l-lactic acid (≥99.2%) in a homofermentative manner from xylose under thermophilic conditions. Strain QU 50 was isolated from Egyptian fertile soil and identified as Enterococcus faecium QU 50 by analyzing its sugar fermentation pattern and 16S rRNA gene sequence. Enterococcus faecium QU 50 fermented xylose efficiently to produce lactic acid over wide pH (6.0-10.0) and temperature ranges (30-52°C), with a pH of 6.5 and temperature of 50°C being optimal. To our knowledge, this is the first report of homofermentative lactic acid production from xylose by a thermophilic lactic acid bacterium.
将木质纤维素材料用于商业目的生产光学纯乳酸受到若干困难的阻碍，包括戊糖的异型发酵以及嗜温乳酸菌的高能量消耗。在此，我们报道了一种新型乳酸菌QU 50菌株，它有潜力在嗜热条件下以同型发酵方式从木糖生产光学纯L-乳酸（≥99.2%）。QU 50菌株从埃及肥沃土壤中分离得到，通过分析其糖发酵模式和16S rRNA基因序列鉴定为粪肠球菌QU 50。粪肠球菌QU 50在较宽的pH范围（6.0 - 10.0）和温度范围（30 - 52°C）内有效地将木糖发酵生成乳酸，最适pH为6.5，最适温度为50°C。据我们所知，这是嗜热乳酸菌从木糖同型发酵生产乳酸的首次报道。
