Nakano Toshiaki, Yoshino Takashi, Fujimura Takao, Arai Satoru, Mukuno Akira, Sato Naoya, Katsuoka Kensei
Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Japan.
Acta Derm Venereol. 2015 Sep;95(7):783-6. doi: 10.2340/00015555-2068.
Dermcidin (DCD), an antimicrobial peptide with a broad spectrum of activity against bacteria such as Propionibacterum acnes, is expressed constitutively in sweat in the absence of stimulation due to injury or inflammation. The aim of this study was to determine the relationship between DCD expression and acne vulgaris associated with P. acnes. The antimicrobial activity of recombinant full-length DCD (50 μg/ml) was 97% against Escherichia coli and 100% against Staphylococcus aureus. Antimicrobial activity against P. acnes ranged from 68% at 50 μg/ml DCD to 83% at 270 μg/ml DCD. DCD concentration in sweat from patients with acne vulgaris (median 9.8 μg/ml, range 6.9-95.3 μg/ml) was significantly lower than in healthy subjects (median 136.7 μg/ml, range 45.4-201.6 μg/ml) (p = 0.001). DCD demonstrated concentration-dependent, but partial, microbicidal activity against P. acnes. These results suggest that reduced DCD concentration in sweat in patients with inflammatory acne may permit proliferation of P. acnes in pilosebaceous units, resulting in progression of inflammatory acne.
皮肤杀菌素（DCD）是一种对痤疮丙酸杆菌等细菌具有广谱活性的抗菌肽，在没有因损伤或炎症引起刺激的情况下，它在汗液中持续表达。本研究的目的是确定DCD表达与痤疮丙酸杆菌相关的寻常痤疮之间的关系。重组全长DCD（50μg/ml）对大肠杆菌的抗菌活性为97%，对金黄色葡萄球菌的抗菌活性为100%。对痤疮丙酸杆菌的抗菌活性在DCD浓度为50μg/ml时为68%，在DCD浓度为270μg/ml时为83%。寻常痤疮患者汗液中的DCD浓度（中位数9.8μg/ml，范围6.9 - 95.3μg/ml）显著低于健康受试者（中位数136.7μg/ml，范围45.4 - 201.6μg/ml）（p = 0.001）。DCD对痤疮丙酸杆菌表现出浓度依赖性但不完全的杀菌活性。这些结果表明，炎症性痤疮患者汗液中DCD浓度降低可能会使痤疮丙酸杆菌在皮脂腺单位中增殖，从而导致炎症性痤疮的进展。
