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外阴阴道念珠菌病特应性受试者对白色念珠菌补体受体3相关蛋白的体液免疫反应。念珠菌感染的新型敏感标志物。

Humoral immune responses to Candida albicans complement receptor 3-related protein in the atopic subjects with vulvovaginal candidiasis. Novel sensitive marker for Candida infection.

作者信息

Paulovičová Ema, Bujdáková Helena, Chupáčová Jarmila, Paulovičová Lucia, Kertys Pavol, Hrubiško Martin

机构信息

Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dubravská cesta 9 Bratislava, Slovakia

Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava, Slovakia, Slovakia.

出版信息

FEMS Yeast Res. 2015 Mar;15(2). doi: 10.1093/femsyr/fou001.

Abstract

In vitro evaluation of specific anti-Candida albicans sera antibodies based on synthetically prepared complement receptor 3-related protein (CR3-RP) mimicking the structure of native complement receptor 3 in a cohort of 72 patients with atopy and recurrent Candida vulvovaginitis (RVC) revealed effective humoral response against Candida CR3-RP. The most significant have been IgM and IgA isotype antibodies (33 and 47% positive cases, respectively). The quantitative evaluation of anti-CR3RP isotype antibodies was confronted with results of commercial ELISA anti-C. albicans antibodies diagnostics based on C. albicans cell wall mannan and β-glucan antigens, the most significant correlation being observed with anti-CR3-RP IgM and anti-β-D-glucan IgM (r(2) = 0.624) followed by isotype IgA (r(2) = 0.381). The immunogenicity and immunoreactivity of CR3RP antigen in RVC patients' sera had been evaluated with regard to the results reached by counterimmunoelectrophoresis and heterogeneous enzyme immunoassay. Obviously, synthetically prepared CR3-RP mimicking the Candida cell-wall-derived structure moiety represents a promising immunological tool not only for Candida serodiagnostics, but also prospectively for follow-up of targeted antifungal therapy and as promising Candida vaccine candidate.

摘要

基于合成制备的模仿天然补体受体3结构的补体受体3相关蛋白(CR3-RP),对72例患有特应性和复发性念珠菌性外阴阴道炎(RVC)的患者队列进行了特异性抗白色念珠菌血清抗体的体外评估,结果显示针对念珠菌CR3-RP存在有效的体液反应。最显著的是IgM和IgA同种型抗体(分别为33%和47%的阳性病例)。抗CR3RP同种型抗体的定量评估与基于白色念珠菌细胞壁甘露聚糖和β-葡聚糖抗原的商业ELISA抗白色念珠菌抗体诊断结果进行了对比,观察到与抗CR3-RP IgM和抗β-D-葡聚糖IgM的相关性最为显著(r(2) = 0.624),其次是同种型IgA(r(2) = 0.381)。已根据对流免疫电泳和异相酶免疫测定的结果评估了CR3RP抗原在RVC患者血清中的免疫原性和免疫反应性。显然,合成制备的模仿念珠菌细胞壁衍生结构部分的CR3-RP不仅是念珠菌血清诊断的一种有前景的免疫学工具,而且在靶向抗真菌治疗的随访方面以及作为有前景的念珠菌疫苗候选物方面也具有前瞻性。

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