大鼠成年组织及R3230AC乳腺肿瘤中PCNA/细胞周期蛋白的基因表达。

Gene expression of PCNA/cyclin in adult tissues and the R3230AC mammary tumor of rat.

作者信息

Liu Y C, Bambara R A

机构信息

Institute of Life Science, National Tsing-Hua University, Hsinchu, Taiwan.

出版信息

Biochem Biophys Res Commun. 1989 Jun 15;161(2):873-82. doi: 10.1016/0006-291x(89)92681-8.

DOI:10.1016/0006-291x(89)92681-8

PMID:2567593

Abstract

We have investigated the gene expression of PCNA (Proliferating Cell Nuclear Antigen)/cyclin in rat tissues and the R3230AC mammary tumor. The steady-state mRNA level of PCNA/cyclin in a tissue is related to the proliferation of the tissue. The observation was confirmed with the results from the studies of the immunoblotting analyses and the DNA polymerase activity measurements. Furthermore, an overexpression of PCNA/cyclin was found in the R3230AC mammary tumor, which is accompanied by an altered PCNA/cyclin gene structure detected with the Southern blot analysis.

摘要

我们研究了大鼠组织和R3230AC乳腺肿瘤中PCNA（增殖细胞核抗原）/细胞周期蛋白的基因表达。组织中PCNA/细胞周期蛋白的稳态mRNA水平与组织的增殖有关。免疫印迹分析和DNA聚合酶活性测量研究结果证实了这一观察结果。此外，在R3230AC乳腺肿瘤中发现PCNA/细胞周期蛋白过表达，同时通过Southern印迹分析检测到PCNA/细胞周期蛋白基因结构改变。