利用C57BL/6J小鼠建立抑郁症慢性束缚应激模型。
Establishment of a chronic restraint stress model of depression with C57BL/6J mice.
作者信息
Sun Nan-nan, Sha Long-ze, Xu Qi
机构信息
State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2015 Feb;37(1):8-11. doi: 10.3881/j.issn.1000-503X.2015.01.002.
OBJECTIVE
To establish a chronic restraint stress (CRS) model of depression,using C57BL/6J mice.
METHODS
Totally 20 C57BL/6J mice were screened by weight,sucrose preference,and the results of open-field test. Then,they were equally randomized into two groups:normal control (NC) group and CRS group. Mice in the CRS group were under restraint 4 hours a day and their behavioral changes were evaluated after 21 days.
RESULTS
The immobility time was significantly longer in CRS mice [(131.70 ± 21.65) s] compared with controls [(68.88 ± 8.43) s] (P=0.0304). CRS mice showed a significant decrease in sucrose preference during the time 0-24 h [(66.21 ±3.24)% vs.(79.46 ± 3.85)%, P=0.0196] and 0-48 h [(73.25 ± 1.50)% vs.(80.20 ± 2.26)%, P=0.0248] compared with controls.
CONCLUSION
The C57BL/6J mice CRS models of depression were successfully established.
目的
使用C57BL/6J小鼠建立抑郁症的慢性束缚应激（CRS）模型。
方法
通过体重、蔗糖偏好和旷场试验结果对20只C57BL/6J小鼠进行筛选。然后，将它们随机分为两组：正常对照组（NC）和CRS组。CRS组小鼠每天束缚4小时，21天后评估其行为变化。
结果
与对照组[(68.88±8.43)s]相比，CRS小鼠的不动时间显著延长(131.70±21.65)s。与对照组相比，CRS小鼠在0 - 24小时[(66.21±3.24)%对(79.46±3.85)%,P=0.0196]和0 - 48小时[(73.25±1.50)%对(80.20±2.26)%,P=0.0248]期间的蔗糖偏好显著降低。
结论
成功建立了C57BL/6J小鼠抑郁症CRS模型。
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