Pals G, Azuma T, Mohandas T K, Bell G I, Bacon J, Samloff I M, Walz D A, Barr P J, Taggart R T
Department of Molecular Biology, School of Medicine, Wayne State University, Detroit, Michigan 48201.
Genomics. 1989 Feb;4(2):137-48. doi: 10.1016/0888-7543(89)90292-9.
A series of six clones containing the entire human pepsinogen C gene (PGC) was identified in a cosmid vector library by using cDNA and oligonucleotide probes. The 10.7-kb PGC gene includes nine exons and exhibits a high degree of sequence identity (60%) with the functionally related pepsinogen A genes. The predicted amino acid sequence was identical with the partial amino-terminal and carboxyl-terminal sequences of purified pepsinogen C. An informative restriction fragment length polymorphism was detected with several restriction enzymes and involved an insertion or deletion of 100 bp of intron sequence located between exons 7 and 8. Evidence that there is only a single PGC gene in humans is presented. The PGC gene and the prolactin gene were regionally localized to 6p21.1-pter by analysis of mouse X human somatic cell hybrids.
通过使用cDNA和寡核苷酸探针，在黏粒载体文库中鉴定出了一系列包含整个人胃蛋白酶原C基因（PGC）的六个克隆。10.7kb的PGC基因包含九个外显子，并且与功能相关的胃蛋白酶原A基因具有高度的序列同一性（60%）。预测的氨基酸序列与纯化的胃蛋白酶原C的部分氨基末端和羧基末端序列相同。用几种限制性内切酶检测到了一种信息丰富的限制性片段长度多态性，其涉及位于外显子7和8之间的100bp内含子序列的插入或缺失。本文提供了人类中仅存在单个PGC基因的证据。通过对小鼠×人类体细胞杂种的分析，将PGC基因和催乳素基因区域定位到6p21.1-pter。
